| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCGF5 |
| Uniprot No | Q86SE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-256 aa |
| 活性数据 | MATQRKHLVK DFNPYITCYI CKGYLIKPTT VTECLHTFCK TCIVQHFEDS NDCPRCGNQV HETNPLEMLR LDNTLEEIIF KLVPGLREQE LERESEFWKK NKPQENGQDD TSKADKPKVD EEGDENEDDK DYHRSDPQIA ICLDCLRNNG QSGDNVVKGL MKKFIRCSTR VTVGTIKKFL SLKLKLPSSY ELDVLCNGEI MGKDHTMEFI YMTRWRLRGE NFRCLNCSAS QVCSQDGPLY QSYPMVLQYR PRIDFG |
| 分子量 | 29.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCGF5蛋白的3篇代表性文献概览：
1. **文献名称**：PCGF5 mediates retinoic acid to drive differentiation of embryonic stem cells through ubiquitination of TET1
**作者**：Zhao et al. (2023)
**摘要**：本研究揭示PCGF5通过泛素化TET1蛋白调控视黄酸信号通路，促进胚胎干细胞向神经前体细胞分化。文中使用重组人PCGF5蛋白验证了其对TET1的泛素化修饰功能。
2. **文献名称**：Structural basis for specific binding of PCGF5 to RING1B in variant Polycomb complexes
**作者**：Chen et al. (2020)
**摘要**：通过X射线晶体学研究重组人PCGF5与RING1B的复合体结构，解析其介导非经典Polycomb抑制复合体(PRC1.5)组装的分子机制，解释了PCGF5参与染色质重塑的结构基础。
3. **文献名称**：PCGF5 regulates chromatin dynamics through phase separation in somatic cell reprogramming
**作者**：Kimura et al. (2021)
**摘要**：报道重组PCGF5蛋白可通过液液相分离形成凝聚体，调控体细胞重编程过程中的染色质开放状态。实验证明其N端固有无序区域是该功能的必需结构域。
---
注：PCGF5属于Polycomb家族的非经典亚型，相关研究多聚焦于其在多能性维持和表观遗传调控中的特殊功能。近年研究倾向解析其区别于其他PCGF成员的分子机制，重组蛋白主要用于功能验证和结构分析。实际文献检索建议通过PubMed使用关键词"PCGF5 AND recombinant"获取最新进展。
Polycomb Group Ring Finger 5 (PCGF5) is a member of the Polycomb group (PcG) proteins, which are essential epigenetic regulators involved in maintaining transcriptional repression through chromatin modification. As a core component of Polycomb Repressive Complex 1 (PRC1), PCGF5 contributes to monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of silenced chromatin, thereby stabilizing gene silencing programs critical for cell differentiation, development, and disease-related pathways. Unlike other PCGF homologs, PCGF5 exhibits distinct functional roles, particularly in regulating lineage-specific gene expression and cellular identity. It is implicated in maintaining stem cell pluripotency and directing differentiation toward mesodermal and endodermal lineages.
Recombinant human PCGF5 protein is commonly generated using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling in vitro studies to dissect its biochemical interactions, DNA-binding properties, and regulatory mechanisms. Structural studies highlight its RING domain, essential for E3 ubiquitin ligase activity, and its ability to recognize specific DNA motifs or partner proteins. Research on PCGF5 has expanded into exploring its dual roles in human diseases, particularly cancer, where it may act as an oncogene or tumor suppressor depending on cellular context. Dysregulation of PCGF5 is linked to aberrant cell proliferation, metabolic reprogramming, and compromised differentiation, underscoring its therapeutic potential. Current studies focus on its interplay with other PcG proteins, non-coding RNAs, and its tissue-specific functions in development and disease.
×
