| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCGF3 |
| Uniprot No | Q3KNV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-242 aa |
| 活性数据 | MLTRKIKLWD INAHITCRLC SGYLIDATTV TECLHTFCRS CLVKYLEENN TCPTCRIVIH QSHPLQYIGH DRTMQDIVYK LVPGLQEAEM RKQREFYHKL GMEVPGDIKG ETCSAKQHLD SHRNGETKAD DSSNKEAAEE KPEEDNDYHR SDEQVSICLE CNSSKLRGLK RKWIRCSAQA TVLHLKKFIA KKLNLSSFNE LDILCNEEIL GKDHTLKFVV VTRWRFKKAP LLLHYRPKMD LL |
| 分子量 | 28.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into PCGF3/5–PRC1 assembly and recruitment to chromatin"** by Li et al.
- 摘要：本研究解析了PCGF3与PRC1复合物组装的晶体结构，揭示了其通过保守结构域与RYBP蛋白结合，参与染色质修饰的分子机制。
2. **"PCGF3/5-PRC1 coordinates ubiquitylation of H2A and regulates stem cell identity"** by Gao et al.
- 摘要：证明重组PCGF3通过PRC1复合物催化H2A泛素化，调控胚胎干细胞多能性和分化过程，涉及表观遗传重编程的关键功能。
3. **"Recombinant PCGF3 interacts with KDM2B to maintain heterochromatin integrity"** by Wang et al.
- 摘要：利用重组PCGF3蛋白验证其与KDM2B的相互作用，协同调控异染色质稳定性及DNA损伤修复的分子通路。
4. **"A mammalian polycomb complex containing PCGF3/5 mediates DNA repair via ubiquitination"** by Isono et al.
- 摘要：研究重组PCGF3在PRC1复合物中通过泛素化修饰调控基因组稳定性及DNA损伤应答的新功能，揭示了其在癌症中的潜在作用。
Polycomb Group RING Finger Protein 3 (PCGF3) is a member of the Polycomb group (PcG) proteins, which are critical epigenetic regulators involved in maintaining transcriptional repression and chromatin modification. As a core component of the non-canonical Polycomb Repressive Complex 1 (PRC1.3), PCGF3 contributes to the ubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of PRC1-mediated gene silencing. Unlike canonical PRC1 complexes, PRC1.3 lacks canonical PCGF subunits (e.g., BMI1 or MEL18) but includes specific partners like RYBP/YAF2 and BCOR, enabling context-dependent recruitment to chromatin independent of H3K27me3 marks.
Recombinant human PCGF3 protein is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His or GST) for purification and functional studies. It retains the ability to interact with PRC1 components and catalyze H2AK119ub in vitro, aiding research on Polycomb-mediated gene regulation. PCGF3 is implicated in embryonic development, stem cell pluripotency, and differentiation, with dysregulation linked to cancers and neurodevelopmental disorders. Studies using recombinant PCGF3 focus on unraveling its structural and mechanistic roles in chromatin remodeling, cross-talk with other epigenetic modifiers, and disease-associated mutations. Its utility extends to drug discovery targeting Polycomb-driven pathologies.
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