| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDH21 |
| Uniprot No | Q96JP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745 aa |
| 活性数据 | MRRCRWAALALGLLRLCLAQANFAPHFFDNGVGSTNGNMALFSLPEDTPVGSHVYTLNGTDPEGDPISYHISFDPSTRSVFSVDPTFGNITLVEELDREREDEIEAIISISDGLNLVAEKVVILVTDANDEAPRFIQEPYVALVPEDIPAGSIIFKVHAVDRDTGSGGSVTYFLQNLHSPFAVDRHSGVLRLQAGATLDYERSRTHYITVVAKDGGGRLHGADVVFSATTTVTVNVEDVQDMAPVFVGTPYYGYVYEDTLPGSEVLKVVAMDGDRGKPNRILYSLVNGNDGAFEINETSGAISITQSPAQLQREVYELHVQVTEMSPAGSPAAQATVPVTIRIVDLNNHPPTFYGESGPQNRFELSMNEHPPQGEILRGLKITVNDSDQGANAKFNLQLVGPRGIFRVVPQTVLNEAQVTIIVENSAAIDFEKSKVLTFKLLAVEVNTPEKFSSTADVVIQLLDTNDNVPKFDSLYYVARIPENAPGGSSVVAVTAVDPDTGPWGEVKYSTYGTGADLFLIHPSTGLIYTQPWASLDAEATARYNFYVKAEDMEGKYSVAEVFITLLDVNDHPPQFGKSVQKKTMVLGTPVKIEAIDEDAEEPNNLVDYSITHAEPANVFDINSHTGEIWLKNSIRSLDALHNITPGRDCLWSLEVQAKDRGSPSFSTTALLKIDITDAEVRRLRYMKNSNFPGTTKSVRKPKFKPKKPHSSQGLFLHPHCEIALFNLSNVNLYSRVFQGAAQAS |
| 分子量 | 107.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例文献，建议通过学术数据库(如PubMed、Google Scholar)查询真实论文：
1. **文献名称**: "PCDH21 is critical for photoreceptor outer segment structure in the mammalian retina"
**作者**: Burgess, R. et al.
**摘要**: 研究揭示了PCDH21蛋白在视网膜光感受器外段发育中的关键作用，其缺失导致结构异常与视觉功能障碍。
2. **文献名称**: "CRX-directed transcriptional regulation of the protocadherin gene PCDH21 during retinal development"
**作者**: Wang, Y. & Chen, S.
**摘要**: 探讨了转录因子CRX如何调控PCDH21在视网膜发育中的表达，及其对光感受器分化的影响。
3. **文献名称**: "A truncating mutation in PCDH21 associated with recessive retinal degeneration"
**作者**: Smith, J.K. et al.
**摘要**: 首次报道了PCDH21基因纯合突变导致隐性遗传性视网膜变性，证实其与人类疾病的相关性。
4. **文献名称**: "Recombinant PCDH21 protein purification and functional analysis in cell adhesion assays"
**作者**: Kumar, A. & Patel, R.
**摘要**: 描述了在HEK293细胞中重组表达PCDH21蛋白的方法，并验证其促进同源性细胞黏附的功能。
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**注意**：上述内容为模拟示例，实际文献需通过学术渠道检索。建议结合关键词“PCDH21” “recombinant” “retina” “protocadherin”等查找最新研究。
**Background of Recombinant Human Protocadherin-21 (PCDH21) Protein**
Protocadherin-21 (PCDH21) is a member of the protocadherin family, a subgroup of calcium-dependent cell adhesion molecules within the cadherin superfamily. These proteins play critical roles in neural development, synaptic specificity, and cellular recognition. PCDH21 is encoded by the *PCDH21* gene located on human chromosome 10 and is predominantly expressed in the retina and brain, suggesting its importance in neuronal and photoreceptor cell functions.
Structurally, PCDH21 contains multiple extracellular cadherin domains that mediate calcium-dependent homophilic interactions, a transmembrane domain, and a cytoplasmic region involved in intracellular signaling. Its precise biological mechanisms remain under investigation, but studies suggest it may regulate photoreceptor outer segment maintenance and synaptic connectivity in the retina. Dysregulation of PCDH21 has been tentatively linked to retinal degenerative diseases.
Recombinant human PCDH21 protein is engineered using in vitro expression systems (e.g., mammalian or insect cells) to mimic its native form, enabling functional studies. Researchers utilize this protein to explore its role in cell adhesion, signaling pathways, and disease mechanisms, aiming to uncover therapeutic targets for neurological or ocular disorders. Current challenges include elucidating its binding partners and tissue-specific functions, highlighting the need for further research.
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