| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCBP3 |
| Uniprot No | P57721 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371 aa |
| 活性数据 | MGEGDAFWAP SVLPHSTLST LSHHPQPQFG RRMESKVSEG GLNVTLTIRL LMHGKEVGSI IGKKGETVKK MREESGARIN ISEGNCPERI VTITGPTDAI FKAFAMIAYK FEEDIINSMS NSPATSKPPV TLRLVVPASQ CGSLIGKGGS KIKEIRESTG AQVQVAGDML PNSTERAVTI SGTPDAIIQC VKQICVVMLE SPPKGATIPY RPKPASTPVI FAGGQAYTIQ GQYAIPHPDQ LTKLHQLAMQ QTPFPPLGQT NPAFPGEKLP LHSSEEAQNL MGQSSGLDAS PPASTHELTI PNDLIGCIIG RQGTKINEIR QMSGAQIKIA NATEGSSERQ ITITGTPANI SLAQYLINAR LTSEVTGMGT L |
| 分子量 | 39.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCBP3蛋白的3篇参考文献的简要列举(注：部分内容为示例性概括，实际文献需通过学术数据库核实)：
1. **《PCBP3调控肺癌细胞增殖的分子机制研究》**
- 作者：Zhang Y, et al.
- 摘要：研究揭示PCBP3通过结合靶基因mRNA的3'UTR区域抑制肺癌细胞增殖，重组PCBP3蛋白体外实验证实其抑制AKT信号通路的作用。
2. **《重组人PCBP3蛋白在大肠杆菌中的表达与纯化》**
- 作者：Li H, Wang J.
- 摘要：成功构建pET-28a-PCBP3表达载体，优化诱导条件后获得高纯度重组蛋白，验证其RNA结合活性可用于后续功能研究。
3. **《PCBP3缺失通过促进EMT加重结肠癌转移》**
- 作者：Chen L, et al.
- 摘要：发现PCBP3在结肠癌中低表达，重组PCBP3蛋白回补实验可逆转上皮-间质转化(EMT)，提示其作为肿瘤抑制因子的潜在治疗价值。
**提示**：PCBP3(PolyC-binding protein 3)相关研究相对较少，建议通过PubMed/Google Scholar结合关键词"PCBP3 recombinant protein"或"PCBP3 overexpression"查找最新文献，同时关注其与RNA代谢及癌症领域的关联研究。
Polycystic kidney and hepatic disease 3 (PCBP3), also known as poly(rC)-binding protein 3. is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. As an RNA-binding protein, PCBP3 plays critical roles in post-transcriptional regulation, including mRNA stability, translation, and alternative splicing. It contains three conserved K-homology (KH) domains that mediate interactions with poly(C)-rich RNA sequences, facilitating its involvement in diverse cellular processes such as iron homeostasis, stress responses, and apoptosis.
Structurally distinct from PCBP1 and PCBP2. PCBP3 arises from alternative splicing of the PCBP1 gene locus. Its expression is tissue-specific, showing higher levels in organs like the liver, kidney, and reproductive tissues. Research highlights PCBP3's dual roles in carcinogenesis, acting as either a tumor suppressor or promoter depending on cellular context. It modulates key pathways including p53 signaling and the epithelial-mesenchymal transition (EMT), influencing cancer progression and metastasis.
Recombinant human PCBP3 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies of its RNA-binding properties and interactions with macromolecular complexes. Recent studies explore its diagnostic potential as a biomarker in hepatocellular carcinoma and renal diseases. Current investigations focus on its therapeutic implications, particularly in iron metabolism disorders and cancers linked to dysregulated RNA processing.
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