| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4K1 |
| Uniprot No | Q8NGD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MAHTNESMVSEFVLLGLSNSWGLQLFFFAIFSIVYVTSVLGNVLIIVIISFDSHLNSPMY FLLSNLSFIDICQSNFATPKMLVDFFIERKTISFEGCMAQIFVLHSFVGSEMMLLVAMAY DRFIAICKPLHYSTIMNRRLCVIFVSISWAVGVLHSVSHLAFTVDLPFCGPNEVDSFFCD LPLVIELACMDTYEMEIMTLTNSGLISLSCFLALIISYTIILIGVRCRSSSGSSKALSTL TAHITVVILFFGPCIYFYIWPFSRLPVDKFLSVFYTVCTPLLNPIIYSLRNEDVKAAMWK LRNRHVNSWKN |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4K1蛋白的模拟参考文献示例(请注意，OR4K1相关研究较少，部分内容为合理推测，建议通过学术数据库核实具体文献)：
1. **《Functional characterization of the olfactory receptor OR4K1 using recombinant expression in HEK293 cells》**
- 作者：Smith A, et al.
- 摘要：本研究通过重组技术在HEK293细胞中表达OR4K1蛋白，结合荧光报告系统鉴定其对特定挥发性分子的反应，证实其作为嗅觉受体的配体激活特性。
2. **《Optimization of membrane protein production: Case study of human OR4K1 in insect cell systems》**
- 作者：Zhang L, et al.
- 摘要：探讨在昆虫细胞中高效表达重组OR4K1蛋白的条件优化，包括密码子优化和去垢剂筛选，以提高蛋白产率并用于结构生物学研究。
3. **《OR4K1 as a potential biomarker in nasal epithelium-derived exosomes: Recombinant protein-assisted detection》**
- 作者：Wang Y, et al.
- 摘要：利用重组OR4K1蛋白制备特异性抗体，开发鼻咽分泌物外泌体中OR4K1的检测方法，评估其在嗅觉功能障碍中的诊断价值。
4. **《Structural insights into OR4K1 via cryo-EM using a stabilized recombinant variant》**
- 作者：Jones R, et al.
- 摘要：通过重组表达并修饰OR4K1蛋白的胞内区，获得稳定构象，利用冷冻电镜解析其三维结构，揭示其与典型GPCR的差异。
**提示**：上述文献为模拟示例，实际研究中OR4K1的报道较少。建议在PubMed、Google Scholar等平台以“OR4K1 recombinant”、“OR4K1 expression”为关键词检索最新文献，或关注嗅觉受体家族整体研究中对特定成员(如OR4K1)的提及。
Recombinant human OR4K1 protein is a genetically engineered form of the olfactory receptor 4K1 (OR4K1), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are critical for odorant detection and signal transduction, linking extracellular chemical cues to intracellular signaling cascades via interactions with odorant molecules. OR4K1. classified under the OR4K subfamily, is encoded by the *OR4K1* gene located on human chromosome 14. Though its precise physiological role remains unclear, it is hypothesized to participate in odor recognition, though some studies suggest ORs may have non-olfactory functions in tissues like the brain, heart, or immune system.
Recombinant OR4K1 is typically produced in heterologous expression systems (e.g., HEK293 or insect cell lines) to overcome challenges in purifying native ORs, which are membrane-bound and low in abundance. The protein’s structure includes seven transmembrane domains, characteristic of GPCRs, with extracellular loops critical for ligand binding. Functional studies often fuse OR4K1 with reporter systems (e.g., luciferase) to assay activation or employ cryo-EM for structural insights.
Research on recombinant OR4K1 focuses on elucidating its ligand specificity, signaling mechanisms, and potential biomedical applications, such as developing biosensors or therapeutic targets. However, functional deorphanization (identifying its natural ligands) remains a key hurdle, limiting mechanistic understanding. This protein also serves as a model for studying GPCR dynamics and membrane protein engineering, contributing to broader advancements in receptor biology and drug discovery.
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