关键信息
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基因名
PMEL
- 应用
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别名
PMEL；D12S53E；PMEL17；SILV；Melanocyte Protein PMEL
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40967
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表达区间
1-661aa
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氨基酸序列
MDLVLKRCLLHLAVIGALLAVGATKVPRNQDWLGVSRQLRTKAWNRQLYP EWTEAQRLDCWRGGQVSLKVSNDGPTLIGANASFSIALNFPGSQKVLPDG QVIWVNNTIINGSQVWGGQPVYPQETDDACIFPDGGPCPSGSWSQKRSFV YVWKTWGQYWQVLGGPVSGLSIGTGRAMLGTHTMEVTVYHRRGSRSYVPL AHSSSAFTITDQVPFSVSVSQLRALDGGNKHFLRNQPLTFALQLHDPSGY LAEADLSYTWDFGDSSGTLISRALVVTHTYLEPGPVTAQVVLQAAIPLTS CGSSPVPGTTDGHRPTAEAPNTTAGQVPTTEVVGTTPGQAPTAEPSGTTS VQVPTTEVISTAPVQMPTAESTGMTPEKVPVSEVMGTTLAEMSTPEATGM TPAEVSIVVLSGTTAAQVTTTEWVETTARELPIPEPEGPDASSIMSTESI TGSLGPLLDGTATLRLVKRQVPLDCVLYRYGSFSVTLDIVQGIESAEILQ AVPSGEGDAFELTVSCQGGLPKEACMEISSPGCQPPAQRLCQPVLPSPAC QLVLHQILKGGSGTYCLNVSLADTNSLAVVSTQLIMPGQEAGLGQVPLIV GILLVLMAVVLASLIYRRRLMKQDFSVPQLPHSSSHWLRLPRIFCSCPIG ENSPLLSGQQV
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分子量
97 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PMEL (Premelanosome Protein) is a crucial protein implicated in the formation of melanosomes, which are organelles responsible for the synthesis and storage of melanin in melanocytes. Understanding PMEL and its role in melanin metabolism is of significant interest not only for basic research in cell biology but also for clinical implications in disorders related to pigmentation, such as vitiligo, albinism, and melanoma. PMEL undergoes a complex series of post-translational modifications, leading to its oligomerization and subsequent incorporation into melanosomes. These processes are vital for the proper development of melanin pigmentation, and any disruption can lead to abnormal skin or hair pigmentation. Recent studies have utilized recombinant DNA technology to express and purify PMEL proteins, enabling detailed studies of their structural and functional properties. This innovative approach allows researchers to dissect the molecular mechanisms underlying PMEL’s role in melanin synthesis, paving the way for potential therapeutic strategies to address pigmentation disorders and enhance our understanding of cellular physiology associated with melanosome biogenesis and function. Thus, research on PMEL restructuring through recombinant protein methodologies represents a promising frontier in both basic science and translational medicine, enhancing our understanding of pigmentation and its associated pathologies.
