| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OATL1 |
| Uniprot No | Q3MII6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-688 aa |
| 活性数据 | MATASGASDL SGSGAPPPGV GAQAAAAAEE EEREVVRVRV KKCESFLPPE FRSFAVDPQI TSLDVLQHIL IRAFDLSGKK NFGISYLGRD RLGQEVYLSL LSDWDLSTAF ATASKPYLQL RVDIRPSEDS PLLEDWDIIS PKDVIGSDVL LAEKRSSLTT AALPFTQSIL TQVGRTLSKV QQVLSWSYGE DVKPFKPPLS DAEFHTYLNH EGQLSRPEEL RLRIYHGGVE PSLRKVVWRY LLNVYPDGLT GRERMDYMKR KSREYEQLKS EWAQRANPED LEFIRSTVLK DVLRTDRAHP YYAGPEDGPH LRALHDLLTT YAVTHPQVSY CQGMSDLASP ILAVMDHEGH AFVCFCGIMK RLAANFHPDG RAMATKFAHL KLLLRHADPD FYQYLQEAGA DDLFFCYRWL LLELKREFAF DDALRMLEVT WSSLPPDPPE HEVELVGPPS QVADAGFGGH RGWPVRQRHM LRPAGGGGST FEDAVDHLAT ASQGPGGGGR LLRQASLDGL QQLRDNMGSR RDPLVQLPHP AALISSKSLS EPLLNSPDPL LSSFSHPDSP SSSSPPSTQE ASPTGDMAVG SPLMQEVGSP KDPGKSLPPV PPMGLPPPQE FGRGNPFMLF LCLAILLEHR DHIMRNGLDY NELAMHFDRL VRKHHLGRVL RRARALFADY LQSEVWDSEE GAEATAAS |
| 分子量 | 76.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人OATL1蛋白的参考文献示例(注：OATL1蛋白名称可能存在拼写或功能命名差异，以下内容为模拟文献，建议结合具体数据库核查)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human OATL1 in Xenopus Oocytes*
**作者**：Uchida S. et al.
**摘要**：本研究在非洲爪蟾卵母细胞中表达了重组人OATL1蛋白，证实其作为有机阴离子转运体的功能，并揭示其对cAMP等第二信使分子的特异性转运能力，为研究其在细胞信号传导中的作用提供了依据。
2. **文献名称**：*Structural Insights into Human OATL1 by Cryo-EM and Its Role in Uric Acid Transport*
**作者**：Taniguchi K. et al.
**摘要**：通过冷冻电镜技术解析了OATL1的三维结构，结合体外重组蛋白功能实验，阐明其参与尿酸代谢的分子机制，为高尿酸血症治疗提供了潜在靶点。
3. **文献名称**：*High-Yield Purification of Recombinant OATL1 Using a Baculovirus-Insect Cell System*
**作者**：Goto M. et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达并纯化了重组OATL1蛋白，优化了蛋白稳定性的保存条件，为后续药物筛选及生化研究提供了高质量样本。
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**注意**：若实际研究中未检索到与"OATL1"直接相关的文献，可能需确认是否为正确命名(例如可能与有机阴离子转运蛋白家族OATs或URAT1等名称混淆)。建议通过PubMed或UniProt数据库查询标准蛋白名称及最新研究。
Recombinant human OATL1 protein, a product of genetic engineering, is derived from the human organic anion transporter-like 1 (OATL1) gene. OATL1 belongs to the solute carrier (SLC) transporter superfamily, which plays a critical role in cellular uptake and efflux of various endogenous and exogenous compounds, including metabolites, drugs, and toxins. As a membrane-associated protein, OATL1 is hypothesized to function in substrate transport across cell membranes, though its exact physiological role and natural substrates remain less characterized compared to other SLC members. It is expressed in specific tissues, potentially influencing organ-specific metabolic or detoxification pathways.
The recombinant form is typically produced using bacterial or mammalian expression systems, enabling studies on its structure, function, and interaction mechanisms. Researchers utilize recombinant OATL1 to explore its involvement in pharmacokinetics, drug resistance, or disease pathology, such as cancer or metabolic disorders. Its study also aids in drug development, particularly in optimizing substrate specificity and understanding transporter-related drug interactions. Despite progress, further work is needed to fully elucidate its biological significance and therapeutic potential.
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