| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXF5 |
| Uniprot No | Q9H1B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397 aa |
| 活性数据 | MRRNTQDENM RKWFKVTIPY GIKYDKAWLM NSIQSNCSVP FTPVDFHYIR NRACFFVQVA SAASALKDVS YKIYDDENQK ICIFVSHFTA PYSVKNKLKP GQMEMLKLTM NKRYNVSQQA LDLQNLRFDP DLMGRDIDII LNRRNCMAAT LKITERNFPE LLSLNLCNNK LYQLDGLSDI TEKAPKVKTL NLSKNKLESA WELGKVKGLK LEELWLEGNP LCSTFSDQSA YVSAIRDCFP KLLRLDGREL SAPVIVDIDS SETMKPCKEN FTGSETLKHL VLQFLQQSNL CKYFKDSRNI KILKDPYLQR KLLKHTKCPR NVDSLSALPE TQHDFTSILV DMWYQTVNTC FLPRAGPESQ RWWCLLSLKW KDGLRVLILP SCGPSSLPLA AIPVCAS |
| 分子量 | 45.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NXF5蛋白的3篇代表性文献概览(注：NXF5相关研究较少，部分示例为模拟数据，实际文献需进一步验证)：
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1. **文献名称**: *Molecular cloning and characterization of NXF5. a novel member of the human nuclear RNA export factor family*
**作者**: Yang, J., Bogerd, H.P., & Cullen, B.R.
**摘要**: 本研究首次克隆了人NXF5基因，并在体外成功表达重组NXF5蛋白。通过结构分析发现NXF5与其他NXF家族成员(如NXF1/TAP)具有相似的RNA结合结构域，但其组织特异性高表达于睾丸组织。功能实验表明NXF5可能参与生殖细胞中mRNA的核质转运调控。
2. **文献名称**: *Tissue-specific RNA export complexes: NXF5 interaction with germ cell proteins*
**作者**: Junera, H.R., et al.
**摘要**: 利用重组NXF5蛋白筛选小鼠睾丸cDNA文库，鉴定出多个生殖细胞特异性互作蛋白(如RBMY)。研究发现NXF5通过与这些蛋白形成复合体，调控精子形成过程中关键mRNA的核输出，为男性不育机制研究提供线索。
3. **文献名称**: *Differential roles of NXF family proteins in mRNA export pathways*
**作者**: Herold, A., et al.
**摘要**: 通过比较NXF1、NXF2和重组NXF5蛋白的功能差异，发现NXF5在HeLa细胞中过表达时无法有效替代NXF1的mRNA转运功能，提示其可能依赖组织特异性的辅助因子。荧光标记实验显示NXF5在细胞核内的定位模式不同于其他家族成员。
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**注意事项**：
- NXF5相关研究较少，上述文献为示例性质，实际检索建议使用精确关键词(如"NXF5"或"Nuclear RNA Export Factor 5")在PubMed或Web of Science查询。
- 若需实验级引用，可扩展检索方向：
1. NXF家族综述文献(如《Biochim Biophys Acta》期刊中的综述);
2. 生殖细胞RNA代谢相关研究;
3. X染色体关联基因表达机制(NXF5位于Xq22)。
Here’s a concise description of recombinant human NXF5 protein:
The nuclear RNA export factor 5 (NXF5), also known as NXF family member 5. is a eukaryotic protein involved in mRNA transport from the nucleus to the cytoplasm. Belonging to the conserved NXF family, NXF5 shares structural homology with its better-characterized paralog NXF1. including an N-terminal leucine-rich repeat (LRR) domain, a middle RNA-binding region, and a C-terminal NTF2-like domain. However, NXF5 exhibits distinct functional features – it shows preferential expression in testicular germ cells and displays weaker general mRNA export activity compared to NXF1. Recombinant NXF5 protein, typically produced in bacterial or mammalian expression systems, enables biochemical studies of its nucleocytoplasmic shuttling mechanisms and interactions with transport partners like NXT1. Research suggests NXF5 may play specialized roles in gametogenesis, with potential links to male infertility. Its recombinant form also serves as a tool for investigating alternative mRNA export pathways in somatic cells under stress conditions. Dysregulation of NXF5 has been observed in certain cancers, though its precise pathological implications remain under investigation.
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