| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUPL1 |
| Uniprot No | Q9BVL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-599 aa |
| 活性数据 | MSTGFSFGSG TLGSTTVAAG GTSTGGVFSF GTGASSNPSV GLNFGNLGST STPATTSAPS SGFGTGLFGS KPATGFTLGG TNTGIATTIT TGLTLGTPAT TSAATTGFSL GFNKPAASAT PFALPITSTS ASGLTLSSAL TSTPAASTGF TLNNLGGTTA TTTTASTGLS LGGALAGLGG SLFQSTNTGT SGLGQNALGL TLGTTAATST AGNEGLGGID FSSSSDKKSD KTGTRPEDSK ALKDENLPPV ICQDVENLQK FVKEQKQVQE EISRMSSKAM LKVQEDIKAL KQLLSLAANG IQRNTLNIDK LKIETAQELK NAEIALRTQK TPPGLQHEYA APADYFRILV QQFEVQLQQY RQQIEELENH LATQANNSHI TPQDLSMAMQ KIYQTFVALA AQLQSIHENV KVLKEQYLGY RKMFLGDAVD VFETRRAEAK KWQNTPRVTT GPTPFSTMPN AAAVAMAATL TQQQQPATGP QPSLGVSFGT PFGSGIGTGL QSSGLGSSNL GGFGTSSGFG CSTTGASTFG FGTTNKPSGS LSAGFGSSST SGFNFSNPGI TASAGLTFGV SNPASAGFGT GGQLLQLKKP PAGNKRGKR |
| 分子量 | 60.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人NUPL1蛋白相关的参考文献示例(注：文献信息为模拟示例，实际引用需核实)：
---
1. **标题**: *"Cloning, Expression, and Purification of Recombinant Human NUPL1 in Escherichia coli"*
**作者**: Zhang Y et al.
**摘要**: 本研究成功在大肠杆菌中克隆并表达了重组人NUPL1蛋白，优化了表达条件并利用亲和层析纯化获得高纯度蛋白。功能分析表明，重组NUPL1在体外能结合RNA，提示其可能参与核内RNA转运过程。
---
2. **标题**: *"NUPL1 Interacts with Hepatitis B Virus Core Protein and Facilitates Viral Replication"*
**作者**: Li J et al.
**摘要**: 通过重组NUPL1蛋白的体外互作实验，发现NUPL1直接与乙肝病毒核心蛋白结合，并通过调节宿主细胞核质运输促进病毒基因组组装，为抗病毒治疗提供了潜在靶点。
---
3. **标题**: *"Structural Insights into Human NUPL1 by Cryo-EM and Its Role in Nuclear Pore Complex Assembly"*
**作者**: Chen X et al.
**摘要**: 利用冷冻电镜解析了重组NUPL1蛋白的三维结构，揭示了其与核孔复合体其他组分的相互作用界面，表明NUPL1在维持核膜完整性及核质运输中发挥关键作用。
---
4. **标题**: *"Functional Characterization of NUPL1 in mRNA Export Using Recombinant Protein-Based Assays"*
**作者**: Wang L et al.
**摘要**: 通过体外重建实验证明，重组NUPL1蛋白能与Exportin-5协同作用，促进特定mRNA的核输出，其缺失导致细胞周期调控异常，提示NUPL1在基因表达调控中的重要性。
---
建议通过PubMed或Google Scholar以关键词“NUPL1”、“recombinant NUPL1 protein”、“nuclear pore complex”检索最新文献以获取准确信息。
NUPL1 (Nucleus and Phosphatase-Linked Protein 1) is a human protein encoded by the *NUPL1* gene located on chromosome 13. Though its precise biological functions remain under active investigation, NUPL1 is implicated in nuclear processes, including chromatin organization, RNA metabolism, and gene expression regulation. The protein contains conserved structural domains, such as a putative nuclear localization signal and a phosphatase-associated domain, suggesting roles in signaling pathways involving phosphorylation/dephosphorylation cycles. Recombinant human NUPL1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies in vitro. Researchers utilize it to explore interactions with nuclear proteins, DNA/RNA molecules, or enzymatic activities linked to phosphatase-related mechanisms. Emerging evidence associates NUPL1 dysregulation with diseases, including certain cancers and neurological disorders, though mechanistic insights are limited. Its recombinant form also serves as an antigen for antibody development or a tool for screening molecular binders. Current challenges include elucidating its 3D structure, mapping interaction networks, and clarifying its context-dependent roles in health and disease. Further studies are needed to define NUPL1's therapeutic potential as a biomarker or intervention target.
×
