关键信息
-
基因名
RNF212
- 应用
-
别名
RNF212；Probable E3 SUMO-Protein ligase RNF212
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q495C1
-
表达区间
1-297aa
-
氨基酸序列
MANWVFCNRCFQPPHRTSCFSLTNCGHVYCDACLGKGKKNECLICKAPCRTVLLSKHTDADIQAFFMSIDSLCKKYSRETSQILEFQEKHRKRLLAFYREKISRLEESLRKSVLQIEQLQSMRSSQQTAFSTIKSSVSTKPHGCLLPPHSSAPDRLESMEVDLSPSPIRKSEIAAGPARISMISPPQDGRMGPHLTASFCFIPWLTLSKPPVPGECVISRGSPCFCIDVCPHWLLLLAFSSGRHGELTNSKTLPIYAEVQRAVLFPFQQAEGTLDTFRTPAVSVVFPLCQFERKKSF
-
分子量
60.4 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RNF212, a member of the RBR E3 ubiquitin ligase family, has garnered significant attention in recent years due to its essential role in meiosis and genomic stability. It is primarily involved in the regulation of homologous recombination during gametogenesis, where it facilitates the proper assembly and disassembly of the synaptonemal complex, a critical structure that holds homologous chromosomes together. Mutations in RNF212 have been linked to reproductive disorders and chromosomal abnormalities, indicating its importance in fertility and genomic integrity. Research on recombinant RNF212 protein has focused on elucidating its functional mechanisms, interaction partners, and its regulatory role in both meiosis and mitotic processes. The production of recombinant RNF212 allows for biochemical assays that can dissect its ubiquitination activity and investigate its potential as a therapeutic target in treating infertile conditions or other disorders linked to DNA repair deficiencies. Overall, understanding RNF212's function at a molecular level is crucial for advancing our knowledge of meiosis, as well as its implications in human health and disease.
