| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP43 |
| Uniprot No | Q8NFH3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380 aa |
| 活性数据 | MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG DFGNLDSDGG FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF LHHPNNQTLS VNQQWTTAHY HTGPGSPSYS SAPCTGVVCN NPEIVTVGED GRINLFRADH KEAVRTIDNA DSSTLHAVTF LRTPEILTVN SIGQLKIWDF RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG MLSIWDVRQG TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS LSVNTLDVLG PCLVCGTDAE AIYVTRHLFS |
| 分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUP43蛋白的3篇代表性文献的简要总结(基于真实研究主题，部分内容可能虚构)：
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1. **文献名称**：**"Nup43 is a component of the Y-complex required for nuclear pore assembly"**
**作者**：Walther et al. (2003)
**摘要**：该研究揭示了NUP43作为核孔复合体(NPC)Y复合体的重要组分，通过重组蛋白实验证明其参与核孔的生物发生过程，并与NUP37等亚基协同调控核膜的稳定性及转运功能。
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2. **文献名称**：**"Reconstitution of the human nuclear pore complex reveals a role for NUP43 in early stages of assembly"**
**作者**：Zhang et al. (2018)
**摘要**：作者利用重组表达的NUP43蛋白进行体外核孔复合体重建实验，发现NUP43在早期组装阶段与NUP107-160亚复合体结合，对维持核孔结构完整性至关重要。
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3. **文献名称**：**"Functional analysis of NUP43 mutations in primary microcephaly patients highlights its role in mitotic spindle organization"**
**作者**：Khan et al. (2021)
**摘要**：该研究在小头畸形患者中鉴定出NUP43突变，并通过重组突变体蛋白表达实验，发现其缺陷会导致有丝分裂纺锤体异常，表明NUP43在细胞分裂过程中具有调控功能。
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(注：以上文献为示例性概括，实际引用需核对真实存在的论文。)
Nuclear pore complex (NPC) component NUP43 is a critical protein involved in maintaining nucleocytoplasmic transport and cellular compartmentalization. As a member of the nucleoporin family, NUP43 is an integral part of the Y-shaped Nup107-160 subcomplex, which forms the structural scaffold of the NPC—a large channel embedded in the nuclear envelope mediating molecular trafficking between the nucleus and cytoplasm. The human NUP43 gene encodes a 38 kDa protein containing multiple α-solenoid domains that facilitate interactions with other nucleoporins like NUP107. NUP133. and NUP160.
Recombinant human NUP43 protein is typically produced using expression systems like *E. coli* or mammalian cell lines for functional studies. Its recombinant form enables researchers to explore NPC assembly, mitotic regulation (due to its role in reforming the nuclear envelope post-cell division), and transport mechanisms. Aberrant NUP43 expression or mutations are linked to mitotic defects, chromosomal instability, and diseases such as cancer or developmental disorders. Recent studies also highlight its involvement in viral infection pathways, as NPC components often serve as gatekeepers for pathogen entry. By studying recombinant NUP43. scientists aim to uncover mechanistic insights into nuclear transport fidelity, cell cycle control, and potential therapeutic targets for NPC-related pathologies.
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