| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUCKS1 |
| Uniprot No | Q9H1E3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-243 aa |
| 活性数据 | MSRPVRNRKV VDYSQFQESD DADEDYGRDS GPPTKKIRSS PREAKNKRRS GKNSQEDSED SEDKDVKTKK DDSHSAEDSE DEKEDHKNVR QQRQAASKAA SKQREMLMED VGSEEEQEEE DEAPFQEKDS GSDEDFLMED DDDSDYGSSK KKNKKMVKKS KPERKEKKMP KPRLKATVTP SPVKGKGKVG RPTASKASKE KTPSPKEEDE EPESPPEKKT STSPPPEKSG DEGSEDEAPS GED |
| 分子量 | 27.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUCKS1蛋白的3篇代表性参考文献示例(基于研究领域常见主题)：
1. **文献名称**：*NUCKS1 is a novel regulator of the transcriptional response to DNA damage*
**作者**：Sekimoto T. et al.
**摘要**：研究报道了重组人NUCKS1蛋白在DNA损伤应答中的作用，通过体外表达纯化NUCKS1并结合ChIP实验，发现其与染色质重塑复合物相互作用，调控靶基因转录，提示其在维持基因组稳定性中的功能。
2. **文献名称**：*Structural and functional characterization of the phosphoproteome of NUCKS1*
**作者**：Rappsilber J. et al.
**摘要**：利用重组NUCKS1蛋白进行质谱分析，系统鉴定了其磷酸化修饰位点，并发现细胞周期依赖性激酶(CDK)介导的磷酸化调控NUCKS1的核定位及与DNA修复蛋白的相互作用。
3. **文献名称**：*NUCKS1 promotes cell proliferation in breast cancer via interaction with PI3K/Akt pathway*
**作者**：Dahl E. et al.
**摘要**：通过表达重组NUCKS1蛋白进行体外功能实验，发现其过表达激活PI3K/Akt信号通路，促进乳腺癌细胞增殖，临床样本分析进一步支持NUCKS1作为潜在肿瘤标志物的可能性。
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**说明**：上述文献为示例性概括，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“recombinant NUCKS1 protein”“NUCKS1 function”或“NUCKS1 phosphorylation”查找最新研究。
**Background of Recombinant Human NUCKS1 Protein**
NUCKS1 (Nuclear Ubiquitous Casein and Cyclin-Dependent Kinase Substrate 1) is a highly conserved nuclear protein ubiquitously expressed in eukaryotic cells. It plays multifaceted roles in DNA damage response, transcriptional regulation, and cell cycle progression. Structurally, NUCKS1 contains an acidic domain, an RGC-rich region, and multiple phosphorylation sites, enabling interactions with key molecules like topoisomerases, chromatin remodelers, and cell cycle regulators (e.g., CDK2).
Functionally, NUCKS1 is implicated in maintaining genomic stability by participating in DNA repair pathways, including homologous recombination and non-homologous end joining. It also modulates chromatin structure, influencing gene expression. Dysregulation of NUCKS1 is linked to cancer progression; overexpression correlates with poor prognosis in breast, prostate, and ovarian cancers. Additionally, post-translational modifications (e.g., phosphorylation, ubiquitination) tightly regulate its activity, connecting it to stress responses and cell proliferation.
Recombinant NUCKS1 protein, produced via prokaryotic or eukaryotic expression systems, retains functional epitopes and is widely used in biochemical assays, antibody development, and mechanistic studies. Its purification often involves affinity chromatography tags (e.g., His-tag) for high yield and purity. Research on recombinant NUCKS1 continues to uncover its therapeutic potential, particularly in targeting cancer pathways and understanding chromatin dynamics.
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