| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUB1 |
| Uniprot No | Q9Y5A7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615 aa |
| 活性数据 | MAQKKYLQAK LTQFLREDRI QLWKPPYTDE NKKVGLALKD LAKQYSDRLE CCENEVEKVI EEIRCKAIER GTGNDNYRTT GIATIEVFLP PRLKKDRKNL LETRLHITGR ELRSKIAETF GLQENYIKIV INKKQLQLGK TLEEQGVAHN VKAMVLELKQ SEEDARKNFQ LEEEEQNEAK LKEKQIQRTK RGLEILAKRA AETVVDPEMT PYLDIANQTG RSIRIPPSER KALMLAMGYH EKGRAFLKRK EYGIALPCLL DADKYFCECC RELLDTVDNY AVLQLDIVWC YFRLEQLECL DDAEKKLNLA QKCFKNCYGE NHQRLVHIKG NCGKEKVLFL RLYLLQGIRN YHSGNDVEAY EYLNKARQLF KELYIDPSKV DNLLQLGFTA QEARLGLRAC DGNVDHAATH ITNRREELAQ IRKEEKEKKR RRLENIRFLK GMGYSTHAAQ QVLHAASGNL DEALKILLSN PQMWWLNDSN PETDNRQESP SQENIDRLVY MGFDALVAEA ALRVFRGNVQ LAAQTLAHNG GSLPPELPLS PEDSLSPPAT SPSDSAGTSS ASTDEDMETE AVNEILEDIP EHEEDYLDST LEDEEIIIAE YLSYVENRKS ATKKN |
| 分子量 | 70.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUB1蛋白的3篇代表性文献，内容简要概括：
1. **文献名称**：*"NUB1-mediated proteasomal degradation of ubiquitin-like proteins is crucial for cell proliferation"*
**作者**：Tanaka, K., et al.
**摘要**：研究证明重组人NUB1通过与26S蛋白酶体亚基结合，促进泛素样蛋白(如FAT10和ISG15)的降解，调控细胞周期进程和增殖，揭示其在肿瘤发生中的潜在作用。
2. **文献名称**：*"Structural and functional analysis of NUB1 as a negative regulator in ubiquitin-like modifier pathways"*
**作者**：Hipp, M.S., et al.
**摘要**：通过体外重组表达NUB1蛋白，发现其含有泛素结合结构域(UBX)，可特异性识别并结合FAT10.诱导靶蛋白的蛋白酶体依赖性降解，证实其在泛素-蛋白酶体系统中的负调控功能。
3. **文献名称**：*"Recombinant NUB1 modulates autophagy by promoting ubiquitination of misfolded proteins"*
**作者**：Li, J., et al.
**摘要**：利用重组NUB1蛋白进行体外实验，发现其通过增强错误折叠蛋白的泛素化修饰，促进自噬途径清除蛋白聚集体，为神经退行性疾病治疗提供新机制依据。
注：以上文献名称、作者及内容均为虚拟概括示例，实际研究中建议通过PubMed或Google Scholar以关键词“NUB1 recombinant protein”检索真实文献。
Recombinant human NUB1 (Negative Regulator of Ubiquitin-like Proteins 1) is a protein implicated in the regulation of ubiquitin-proteasome pathways. Initially identified as an interactor of the ubiquitin-like protein NEDD8. NUB1 functions as a scaffold protein that mediates the degradation of ubiquitin-like modifiers (UBLs) by recruiting them to the proteasome. Structurally, it contains ubiquitin-associated (UBA) and ubiquitin-like (UBL) domains, which facilitate interactions with proteasomal components and ubiquitinated substrates. NUB1’s activity modulates cellular protein turnover, influencing processes such as cell cycle progression, stress responses, and apoptosis. Studies highlight its role in neuroprotection, cancer progression, and immune regulation by controlling the stability of key signaling molecules. Recombinant NUB1. produced via expression systems like *E. coli* or mammalian cells, enables mechanistic studies of its binding partners, post-translational modifications, and regulatory networks. Dysregulation of NUB1 has been linked to neurodegenerative diseases (e.g., Parkinson’s) and malignancies, making it a potential therapeutic target. Current research focuses on clarifying its dual roles in promoting or inhibiting degradation, depending on cellular context, and its crosstalk with autophagy pathways. Its recombinant form is critical for structural analysis, inhibitor screening, and functional assays in vitro.
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