| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUAK2 |
| Uniprot No | Q9H093 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-628 aa |
| 活性数据 | MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS SESFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL GDSCFSLTDC QEVTATYRQA LRVCSKLT |
| 分子量 | 69.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NUAK2蛋白的3篇文献示例：
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**1. 文献名称**：*NUAK2 promotes cell migration and induces epithelial-mesenchymal transition in cancer cells*
**作者**：Sakamaki J et al.
**摘要**：本研究通过构建重组人NUAK2蛋白，发现其过表达可激活多种促迁移信号通路，并诱导癌细胞发生上皮-间充质转化(EMT)。实验表明，NUAK2通过磷酸化下游靶蛋白调控肌动蛋白重塑，促进肿瘤侵袭转移。
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**2. 文献名称**：*Structural and functional characterization of the AMPK-related kinase NUAK2*
**作者**：Vazquez-Martin A et al.
**摘要**：文章解析了重组表达的人源NUAK2蛋白激酶结构域的三维结构，揭示其底物结合特异性的分子机制。功能实验表明，NUAK2活性受AMP/ATP水平调节，并参与能量应激条件下的细胞存活调控。
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**3. 文献名称**：*NUAK2 interacts with mitochondrial proteins and regulates oxidative stress response*
**作者**：Lee JH et al.
**摘要**：通过重组NUAK2蛋白进行免疫共沉淀-质谱分析，鉴定了其与线粒体抗氧化酶(如SOD2)的相互作用。研究证实NUAK2通过调控线粒体功能减轻氧化应激损伤，提示其在神经退行性疾病中的潜在作用。
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*注：以上文献信息为模拟示例，实际引用时需以真实文献为准。建议通过PubMed或Google Scholar以“NUAK2 recombinant protein”“NUAK2 function”等关键词检索具体文献。*
NUAK2 (NUAK family kinase 2), also known as SNARK, is a serine/threonine kinase belonging to the AMP-activated protein kinase (AMPK) family. It plays a critical role in cellular stress responses, energy metabolism, and pathways regulating cell proliferation, survival, and motility. NUAK2 is activated under conditions of nutrient deprivation or energy stress, often interacting with upstream regulators like LKB1 and AMPK. Its activity influences phosphorylation events in key signaling pathways, including the Hippo and mTOR pathways, thereby modulating processes such as autophagy, cytoskeletal organization, and epithelial-mesenchymal transition.
Recombinant human NUAK2 protein is produced using expression systems like *E. coli* or mammalian cells, enabling standardized study of its enzymatic activity, substrate interactions, and structural properties. This engineered protein retains kinase functionality, making it valuable for *in vitro* assays, drug screening, and mechanistic studies. Research highlights NUAK2's dual roles in cancer—acting as both a tumor suppressor and promoter depending on context—and its implication in metabolic disorders and fibrosis. Dysregulation of NUAK2 is linked to metastasis, chemoresistance, and abnormal glucose metabolism, positioning it as a potential therapeutic target. Recombinant NUAK2 tools are essential for unraveling its complex biology and developing targeted inhibitors or activators.
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