| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTAN1 |
| Uniprot No | Q96AB6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-310 aa |
| 活性数据 | PLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG LWEKISSPGS |
| 分子量 | 34.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NTAN1蛋白的3篇代表性文献示例(供参考，实际文献需根据具体需求检索)：
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1. **文献名称**：*"Recombinant Human NTAN1: Expression, Purification, and Enzymatic Characterization"*
**作者**：Lee S, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人NTAN1蛋白，确认其具有N端天冬酰胺脱酰胺酶活性，并揭示了其底物特异性依赖于特定氨基酸序列的N末端结构。
2. **文献名称**：*"Structural Insights into the Catalytic Mechanism of NTAN1 in the N-End Rule Pathway"*
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析了NTAN1的三维结构，阐明了其结合底物的关键氨基酸残基及催化机制，为研究其在蛋白质降解中的作用提供结构基础。
3. **文献名称**：*"NTAN1 Regulates Cell Proliferation via Degradation of Cyclin-Dependent Kinase Inhibitors"*
**作者**：Kumar A, et al.
**摘要**：研究发现NTAN1通过N-end rule途径调控细胞周期蛋白抑制因子的稳定性，影响细胞周期进程，提示其在肿瘤发生中的潜在作用。
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**说明**：
- 以上文献为示例，实际研究中需通过PubMed或Google Scholar等平台以“recombinant NTAN1”“human NTAN1 function”等关键词检索最新论文。
- NTAN1的研究多集中于其酶学特性、结构及在蛋白质降解通路(如N-end rule pathway)中的调控作用，部分研究涉及癌症、神经退行性疾病等领域。
建议结合具体研究方向进一步筛选文献!
Recombinant human NTAN1 (N-terminal asparagine amidase 1) protein is a genetically engineered form of the enzyme encoded by the *NTAN1* gene, which plays a critical role in the N-terminal deglycation pathway. NTAN1. a cytosolic enzyme belonging to the amidohydrolase family, specifically cleaves N-terminal asparagine or glutamine residues from proteins, initiating their degradation via the N-end rule pathway—a process regulating protein stability and turnover. This enzymatic activity is essential for maintaining cellular homeostasis, influencing processes such as apoptosis, cell cycle progression, and stress responses. Researchers produce recombinant NTAN1 using expression systems like *E. coli* or mammalian cells, enabling studies on its structure, substrate specificity, and interaction partners. Its dysregulation has been linked to developmental disorders and cancers, making it a focus for therapeutic targeting. Recombinant NTAN1 is widely utilized in biochemical assays, structural biology (e.g., crystallography), and drug discovery to explore mechanisms underlying protein degradation and develop modulators of N-terminal modification pathways. Ongoing research aims to elucidate its regulatory networks and potential applications in diseases associated with proteostatic imbalance.
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