| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5DC2 |
| Uniprot No | Q9H857 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520 aa |
| 活性数据 | MRVESGSAQE RGILLESLST LLEKTTASHE GRAPGNRELT DLLPPEVCSL LNPAAIYANN EISLRDVEVY GFDYDYTLAQ YADALHPEIF STARDILIEH YKYPEGIRKY DYNPSFAIRG LHYDIQKSLL MKIDAFHYVQ LGTAYRGLQP VPDEEVIELY GGTQHIPLYQ MSGFYGKGPS IKQFMDIFSL PEMALLSCVV DYFLGHSLEF DQAHLYKDVT DAIRDVHVKG LMYQWIEQDM EKYILRGDET FAVLSRLVAH GKQLFLITNS PFSFVDKGMR HMVGPDWRQL FDVVIVQADK PSFFTDRRKP FRKLDEKGSL QWDRITRLEK GKIYRQGNLF DFLRLTEWRG PRVLYFGDHL YSDLADLMLR HGWRTGAIIP ELEREIRIIN TEQYMHSLTW QQALTGLLER MQTYQDAESR QVLAAWMKER QELRCITKAL FNAQFGSIFR TFHNPTYFSR RLVRFSDLYM ASLSCLLNYR VDFTFYPRRT PLQHEAPLWM DQLCTGCMKT PFLGDMAHIR |
| 分子量 | 60.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NT5DC2蛋白的示例文献概览(注：以下为虚构示例，实际文献需通过数据库检索确认)：
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1. **文献名称**：*NT5DC2 recombinant protein expression and its role in cancer cell proliferation*
**作者**：Zhang L, et al.
**摘要**：研究报道了人NT5DC2重组蛋白在大肠杆菌中的可溶性表达及纯化，发现该蛋白通过调节AMP代谢抑制肝癌细胞系HepG2的增殖，提示其潜在抑癌功能。
2. **文献名称**：*Structural characterization of human NT5DC2 and its interaction with nucleotides*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了NT5DC2重组蛋白的三维结构，揭示了其核苷酸结合域的关键位点，为靶向药物设计提供了结构基础。
3. **文献名称**：*NT5DC2 modulates neuronal differentiation via purinergic signaling pathways*
**作者**：Kim S, et al.
**摘要**：利用哺乳动物细胞表达的重组NT5DC2蛋白，发现其通过水解胞外ATP影响神经干细胞分化，提示其在神经系统发育中的作用。
4. **文献名称**：*High-throughput screening of NT5DC2 inhibitors using recombinant protein*
**作者**：Chen X, et al.
**摘要**：开发了基于NT5DC2重组酶的活性检测体系，筛选出小分子抑制剂库中的潜在先导化合物，用于代谢性疾病治疗研究。
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**提示**：建议通过PubMed/Google Scholar搜索关键词“NT5DC2 recombinant”、“human NT5DC2 protein”获取真实文献，可重点关注功能机制、结构解析或疾病相关性研究。
**Background of Recombinant Human NT5DC2 Protein**
Recombinant human NT5DC2 (5'-nucleotidase domain-containing protein 2) is a genetically engineered protein belonging to the 5'-nucleotidase family, which plays roles in nucleotide metabolism and cellular signaling. The native NT5DC2 gene encodes a protein with a conserved 5'-nucleotidase domain, though its exact enzymatic activity and substrates remain under investigation. Studies suggest NT5DC2 may regulate cellular processes such as proliferation, differentiation, and apoptosis, potentially influencing pathological conditions like cancer, neurodegenerative diseases, and viral infections.
Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant NT5DC2 enables functional studies by providing a purified, bioactive form. Its structural characterization reveals potential interactions with signaling pathways, including MAPK/ERK, hinting at roles in stress response and oncogenesis. NT5DC2 has garnered interest as a biomarker, with upregulated expression observed in cancers like hepatocellular carcinoma and glioma, correlating with tumor progression and drug resistance. Conversely, its neuroprotective effects in models of Parkinson’s disease highlight therapeutic potential.
Research on recombinant NT5DC2 focuses on elucidating its molecular mechanisms, substrate specificity, and role in disease, offering insights for drug development. Despite incomplete functional understanding, its involvement in critical pathways positions it as a promising target for diagnostics and therapeutics. Ongoing studies aim to clarify its biological significance and harness its potential in precision medicine.
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