| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NT5DC1 |
| Uniprot No | Q5TFE4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455 aa |
| 活性数据 | MAQHFSLAAC DVVGFDLDHT LCRYNLPESA PLIYNSFAQF LVKEKGYDKE LLNVTPEDWD FCCKGLALDL EDGNFLKLAN NGTVLRASHG TKMMTPEVLA EAYGKKEWKH FLSDTGMACR SGKYYFYDNY FDLPGALLCA RVVDYLTKLN NGQKTFDFWK DIVAAIQHNY KMSAFKENCG IYFPEIKRDP GRYLHSCPES VKKWLRQLKN AGKILLLITS SHSDYCRLLC EYILGNDFTD LFDIVITNAL KPGFFSHLPS QRPFRTLEND EEQEALPSLD KPGWYSQGNA VHLYELLKKM TGKPEPKVVY FGDSMHSDIF PARHYSNWET VLILEELRGD EGTRSQRPEE SEPLEKKGKY EGPKAKPLNT SSKKWGSFFI DSVLGLENTE DSLVYTWSCK RISTYSTIAI PSIEAIAELP LDYKFTRFSS SNSKTAGYYP NPPLVLSSDE TLISK |
| 分子量 | 51.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NT5DC1蛋白的3篇代表性文献的简要总结(基于现有研究推测，部分信息可能需核实)：
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1. **文献名称**: "Characterization of recombinant human NT5DC1 as a mitochondrial nucleotidase"
**作者**: Zhang Y, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人NT5DC1蛋白，验证其具有5'-核苷酸酶活性，优先水解嘌呤核苷酸(如AMP)。进一步实验表明NT5DC1定位于线粒体，可能参与线粒体能量代谢调控。
2. **文献名称**: "NT5DC1 promotes lung cancer cell proliferation by stabilizing EGFR"
**作者**: Wang L, et al.
**摘要**: 通过重组NT5DC1蛋白的体外结合实验，发现其能与表皮生长因子受体(EGFR)直接相互作用，抑制EGFR泛素化降解，进而促进非小细胞肺癌细胞的增殖和转移。
3. **文献名称**: "Structural insights into the catalytic mechanism of human NT5DC1"
**作者**: Liu X, et al.
**摘要**: 本研究解析了重组人NT5DC1蛋白的晶体结构(分辨率2.1Å)，揭示了其活性位点的关键氨基酸残基(如His-158和Asp-202)，并通过突变实验验证了其在核苷酸水解中的催化机制。
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**注意**：以上文献信息为模拟示例，实际文献需通过PubMed或Web of Science等平台检索确认。建议使用关键词“NT5DC1 recombinant”“NT5DC1 structure/function”筛查近5年文献。
Recombinant human NT5DC1 (5'-nucleotidase domain-containing protein 1) is a genetically engineered protein derived from the human NT5DC1 gene, which encodes a member of the 5'-nucleotidase family. This enzyme family is implicated in nucleotide metabolism, primarily catalyzing the hydrolysis of nucleoside monophosphates to generate nucleosides and inorganic phosphate. NT5DC1 shares structural homology with other 5'-nucleotidases but exhibits distinct substrate preferences and regulatory mechanisms. It contains conserved catalytic motifs critical for its enzymatic activity, though its exact physiological substrates remain under investigation.
NT5DC1 is broadly expressed in human tissues, including the brain, liver, and immune cells, suggesting roles in cellular homeostasis, energy metabolism, and signaling pathways. Studies link it to purinergic signaling, a system regulating processes like inflammation, neurotransmission, and apoptosis. Emerging evidence associates NT5DC1 dysregulation with pathological conditions, such as cancer progression, neurodegenerative disorders, and metabolic syndromes. For instance, it may influence tumor cell proliferation or neuroprotection by modulating nucleotide pools or interacting with disease-related proteins.
Recombinant NT5DC1 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies, structural analysis, and drug discovery. Its applications span elucidating enzymatic mechanisms, screening inhibitors, and exploring therapeutic targets. Ongoing research aims to clarify its precise biological roles and therapeutic potential in human diseases.
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