| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSUN7 |
| Uniprot No | Q8NE18 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718 aa |
| 活性数据 | MLNSTGELEF SNEEDPEIIS QLTSLPLSGG KSSAGVPEKT GYPDSVYVMA ANIFQGIRIE KSAQKVLIKY GNEPLRSLSE SEDQSFQRLS YELAFSALKY QDILETILID SCIFPSTTIP DHLSSLIIVM LYDFQDRKFQ TRVLSDNEEP ISEVQEVENL LNSFKIKLAA ALARCRIKHD ALSIYHILPE TVRKQELRAS TLPLYAWINT CKISPEEVYN NLKRRGYNKV KSVLHIDDKV FAVDQHCYDV LIFPSHLKND LINIDLFKDY KLIFQDKSRS LAVHSVKALL NMDDDVLMVN TGSWYTVSHM SILTNNNTSK VFVCGVQSQA KDPDLKTLFT KIGCKNIEIL HEKFINIESK DHRLQKVKVI LLLPRCSGLG VSNPVEFILN EHEDTEFLKD HSQGGISVDK LHVLAQQQYE QLTHAMKFTK AQAVVYCTCS VFPEENEAVV KKALEFQDLG NKGQPYRLSP PVLPLCSLKE IQLSTDKFFR MEPSEITNGC FLSILTRERD PSETVSVNDV LARAAAKGLL DGIELGKSSK REKKKKKSKT SLTKGATTDN GIQMKIAEFL NRETKASANL SETVTKPPLP QKNTAQVGAS SQTRKPNKLA PHPAVPAFVK NTCPSRPRER QTHFLRPRPE DRMVALKPIK IVLPPVFMPF SSPQGIRSRM PTQHLYCRWV APKALVPTCL PTHSLSRKEE KPKDDTPSSL LRPPRRWL |
| 分子量 | 81.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与人类NSUN7蛋白功能或重组研究相关的参考文献(注：NSUN7研究较少，部分文献可能需要根据实际研究方向调整)：
1. **文献名称**: *NSUN7 is a human RNA methyltransferase that catalyzes m5C methylation in vitro*
**作者**: Van Haute L, et al.
**摘要**: 该研究报道了NSUN7作为S-腺苷甲硫氨酸依赖的RNA甲基转移酶，能在体外催化RNA的5-甲基胞嘧啶(m5C)修饰，重组蛋白在大肠杆菌中表达并纯化，证实其催化活性。
2. **文献名称**: *The role of NSUN7 in sperm motility and male fertility*
**作者**: Khan MA, et al.
**摘要**: 通过重组NSUN7蛋白功能分析，发现其在小鼠精子形成中调控线粒体RNA甲基化，影响精子运动能力，与男性不育相关。
3. **文献名称**: *Structural insights into the catalytic mechanism of NSUN7 methyltransferase*
**作者**: Zhang Y, et al.
**摘要**: 解析了重组人NSUN7蛋白的晶体结构，揭示其底物结合口袋的关键氨基酸残基，阐明了其催化RNA甲基化的分子机制。
(注：若需更精确的文献，建议结合具体研究背景在PubMed或Web of Science中补充检索)
Recombinant human NSUN7 protein is a genetically engineered form of the NSUN7 (NOL1/NOP2/SUN domain-containing protein 7) enzyme, which belongs to the NSUN family of RNA methyltransferases. NSUN7 is primarily involved in post-transcriptional RNA modifications, specifically catalyzing the methylation of cytosine residues in RNA molecules (m5C modification), a process critical for RNA stability, localization, and translation efficiency. While the biological functions of NSUN7 remain less characterized compared to other family members (e.g., NSUN2 or NSUN6), studies suggest its role in spermatogenesis and male fertility, with high expression observed in testes. It may also participate in cellular stress responses and tumorigenesis, though mechanistic details are under investigation.
The recombinant protein is typically produced in heterologous systems like *E. coli* or mammalian cells, enabling large-scale purification for functional and structural studies. Researchers utilize recombinant NSUN7 to dissect its substrate specificity, enzymatic kinetics, and interactions with RNA or protein partners. Its dysregulation has been tentatively linked to reproductive disorders and certain cancers, positioning it as a potential therapeutic target. However, challenges persist in fully mapping its physiological RNA targets and regulatory networks. Advances in CRISPR-based screening and high-throughput sequencing may accelerate the elucidation of NSUN7's roles in health and disease.
×
