| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSUN5 |
| Uniprot No | Q96P11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-429 aa |
| 活性数据 | GLYAAAAGV LAGVESRQGS IKGLVYSSNF QNVKQLYALV CETQRYSAVL DAVIASAGLL RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA RLKAELARLK VHRGVSRNED LLEVGSRPGP ASQLPRFVRV NTLKTCSDDV VDYFKRQGFS YQGRASSLDD LRALKGKHFL LDPLMPELLV FPAQTDLHEH PLYRAGHLIL QDRASCLPAM LLDPPPGSHV IDACAAPGNK TSHLAALLKN QGKIFAFDLD AKRLASMATL LARAGVSCCE LAEEDFLAVS PSDPRYHEVH YILLDPSCSG SGMPSRQLEE PGAGTPSPVR LHALAGFQQR ALCHALTFPS LQRLVYSTCS LCQEENEDVV RDALQQNPGA FRLAPALPAW PHRGLSTFPG AEHCLRASPE TTLSSGFFVA VIERVEVPR |
| 分子量 | 46.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NSUN5蛋白的3篇参考文献及简要摘要：
1. **文献名称**：Structural insights into human NSUN5-RNA methyltransferase活性机制
**作者**：Li, X. et al.
**摘要**：通过X射线晶体学解析了重组人NSUN5蛋白的结构，揭示了其催化RNA C-甲基化的活性位点特征，并发现其底物偏好性受保守结构域调控。
2. **文献名称**：NSUN5缺失通过影响核糖体RNA修饰促进胶质母细胞瘤侵袭
**作者**：Martínez, F.J. et al.
**摘要**：研究表明，重组NSUN5蛋白介导的28S rRNA甲基化缺失导致核糖体翻译保真度下降，进而促进胶质瘤细胞迁移及肿瘤恶性进展。
3. **文献名称**：重组NSUN5的酶动力学及底物筛选
**作者**：Chen, Z. et al.
**摘要**：通过体外酶活实验验证重组人NSUN5对RNA底物的甲基化活性，筛选出特定序列响应区域，并建立酶促反应动力学模型。
(注：以上文献信息为示例，实际文献需通过学术数据库检索确认。)
NSUN5. a member of the NOL1/NOP2/SUN domain (NSUN) family of methyltransferases, is a conserved RNA-binding protein implicated in post-transcriptional modifications, particularly 5-methylcytosine (m5C) methylation of RNA. It catalyzes the methylation of cytosine residues in ribosomal RNA (rRNA), notably at position C3782 in 28S rRNA, a process critical for ribosome biogenesis and translational fidelity. NSUN5 is localized in the nucleolus and cytoplasm, reflecting its dual role in rRNA maturation and ribosome function.
Initially linked to developmental processes, NSUN5 gained attention for its association with neurological disorders and cancer. In humans, its dysregulation is observed in glioblastoma, where it may act as a tumor suppressor by modulating stress-adapted translation, and in Down syndrome, where reduced expression correlates with intellectual disability. Studies in model organisms, such as *Drosophila* and mice, highlight its importance in lifespan regulation and neuronal function.
Recombinant human NSUN5 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is widely used to study m5C deposition mechanisms, RNA-protein interactions, and structural features. Its crystal structure reveals conserved motifs essential for substrate binding and catalytic activity. Research on NSUN5 continues to explore its therapeutic potential, particularly in diseases linked to translational dysregulation or epigenetic alterations.
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