| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSUN4 |
| Uniprot No | Q96CB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-384 aa |
| 活性数据 | RYKKK WAATEPKFPA VRLALQNFDM TYSVQFGDLW PSIRVSLLSE QKYGALVNNF AAWDHVSAKL EQLSAKDFVN EAISHWELQS EGGQSAAPSP ASWACSPNLR CFTFDRGDIS RFPPARPGSL GVMEYYLMDA ASLLPVLALG LQPGDIVLDL CAAPGGKTLA LLQTGCCRNL AANDLSPSRI ARLQKILHSY VPEEIRDGNQ VRVTSWDGRK WGELEGDTYD RVLVDVPCTT DRHSLHEEEN NIFKRSRKKE RQILPVLQVQ LLAAGLLATK PGGHVVYSTC SLSHLQNEYV VQGAIELLAN QYSIQVQVED LTHFRRVFMD TFCFFSSCQV GELVIPNLMA NFGPMYFCKM RRLT |
| 分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitochondrial assembly"**
作者：Metodiev et al.
摘要：该研究发现NSUN4不仅作为线粒体RNA甲基转移酶修饰12S rRNA，还与MTERF4形成复合物，调控线粒体核糖体组装，对维持线粒体呼吸链功能至关重要。敲除NSUN4导致小鼠胚胎致死及线粒体蛋白质合成缺陷。
2. **"The role of NSUN4 in spermatogenesis and male fertility in mice"**
作者：Lv et al.
摘要：研究揭示NSUN4在小鼠睾丸中高表达，特异性参与精子形成过程中的线粒体RNA修饰。条件性敲除NSUN4引发雄性不育，表现为精子活力下降及线粒体结构异常，提示其与生殖细胞发育密切相关。
3. **"NSUN4 deficiency disrupts the mitochondrial translation machinery and causes cardiomyopathy"**
作者：Richman et al.
摘要：通过心脏特异性敲除模型，发现NSUN4缺失导致心脏线粒体核糖体亚基组装障碍，引发心肌病。研究强调NSUN4在心脏能量代谢中的关键作用，并关联其突变与人类扩张型心肌病的潜在机制。
4. **"Altered NSUN4 expression correlates with tumor progression in hepatocellular carcinoma"**
作者：Zhang et al.
摘要：该临床研究显示，NSUN4在肝癌组织中显著高表达，且与患者不良预后正相关。机制研究表明NSUN4通过增强线粒体翻译能力促进肿瘤细胞增殖，提示其作为癌症治疗靶点的潜力。
NSUN4 (NOP2/Sun RNA methyltransferase family member 4) is a human protein belonging to the S-adenosylmethionine-dependent methyltransferase family, primarily implicated in post-transcriptional RNA modifications. It is a mitochondrial protein critical for mitochondrial ribosome assembly and function. NSUN4 forms a complex with MTERF4. and this interaction is essential for the stability and proper maturation of the large ribosomal subunit (39S) in mitochondria. By catalyzing the methylation of 12S rRNA at position C911. NSUN4 ensures the structural integrity of mitochondrial ribosomes, thereby supporting efficient mitochondrial translation and oxidative phosphorylation.
Recombinant human NSUN4 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to investigate molecular mechanisms of mitochondrial RNA methylation, ribosome biogenesis, and energy metabolism. Dysregulation of NSUN4 has been linked to mitochondrial dysfunction, implicated in diseases such as cancer, neurodegenerative disorders, and male infertility. Structural studies of recombinant NSUN4 have revealed insights into its catalytic domains and substrate-binding specificity, aiding drug development targeting mitochondrial translation defects. Current research also explores its regulatory role in cellular stress responses and potential as a biomarker for mitochondrial diseases. The availability of recombinant NSUN4 facilitates biochemical assays, crystallography, and screening for therapeutic modulators.
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