| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSMCE4A |
| Uniprot No | Q9NXX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385 aa |
| 活性数据 | MSGDSSGRGP EGRGRGRDPH RDRTRSRSRS RSPLSPRSRR GSARERREAP ERPSLEDTEP SDSGDEMMDP ASLEAEADQG LCRQIRHQYR ALINSVQQNR EDILNAGDKL TEVLEEANTL FNEVSRAREA VLDAHFLVLA SDLGKEKAKQ LRSDLSSFDM LRYVETLLTH MGVNPLEAEE LIRDEDSPDF EFIVYDSWKI TGRTAENTFN KTHTFHFLLG SIYGECPVPK PRVDRPRKVP VIQEERAMPA QLRRMEESHQ EATEKEVERI LGLLQTYFRE DPDTPMSFFD FVVDPHSFPR TVENIFHVSF IIRDGFARIR LDQDRLPVIE PVSINEENEG FEHNTQVRNQ GIIALSYRDW EEIVKTFEIS EPVITPSQRQ QKPSA |
| 分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NSMCE4A蛋白的3篇参考文献的简要列举：
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1. **文献名称**：*Structural and functional insights into the role of NSMCE4A in the SMC5/6 complex*
**作者**：Smith, J. et al. (2021)
**摘要**：
该研究通过体外重组表达NSMCE4A蛋白，揭示其与SMC5-SMC6复合体其他亚基的相互作用，证明其在DNA损伤修复中调控复合体构象及酶活性的关键作用，并提出NSMCE4A缺失导致染色体不稳定的机制。
2. **文献名称**：*NSMCE4A deficiency disrupts DNA replication and promotes genome instability in human cells*
**作者**：Wang, L. & Chen, R. (2019)
**摘要**：
通过构建重组NSMCE4A突变体，发现其缺失会阻碍SMC5/6复合体在复制叉上的定位，导致复制压力累积和DNA双链断裂增加，表明NSMCE4A在维持复制完整性及基因组稳定性中的必要功能。
3. **文献名称**：*Recombinant NSMCE4A exhibits ATPase activity critical for SMC5/6 complex function*
**作者**：Garcia, M. et al. (2018)
**摘要**：
研究首次纯化重组人NSMCE4A蛋白并表征其ATP酶活性，证实其依赖DNA刺激的ATP水解能力，支持SMC5/6复合体通过NSMCE4A介导的ATP代谢参与染色体高级结构重塑和同源重组修复。
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注：上述文献为虚拟示例，实际研究中建议通过PubMed、Web of Science等平台检索关键词“NSMCE4A”、“SMC5/6 complex”或“genome stability”获取真实文献。该蛋白在DNA修复复合体中的功能及重组表达策略为当前研究热点。
NSMCE4A (Structural Maintenance of Chromosomes 5/6 Complex Subunit E4A) is a critical component of the SMC5/6 protein complex, which plays essential roles in maintaining genome stability and DNA repair processes. This evolutionarily conserved complex is involved in repairing DNA damage, resolving replication stress, and ensuring proper chromosome segregation during cell division. As a non-SMC regulatory subunit, NSMCE4A contributes to the structural integrity and enzymatic activities of the SMC5/6 complex, particularly in facilitating SUMOylation—a post-translational modification crucial for DNA repair and chromatin remodeling.
Studies suggest that NSMCE4A participates in homologous recombination repair, telomere maintenance, and suppression of illegitimate recombination. Its dysfunction has been linked to genomic instability, developmental disorders, and cancer susceptibility. Recombinant human NSMCE4A protein, typically produced in expression systems like *E. coli* or mammalian cells, enables biochemical characterization of its interactions with SMC5/6 subunits, substrates, and cofactors. This recombinant tool has advanced structural studies, mechanistic investigations of DNA repair pathways, and screening for potential therapeutic agents targeting genome maintenance mechanisms. Recent research also explores NSMCE4A's role in viral defense, as the SMC5/6 complex restricts certain viruses by compacting viral DNA.
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