| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSMCE2 |
| Uniprot No | Q96MF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247 aa |
| 活性数据 | MPGRSSSNSG STGFISFSGV ESALSSLKNF QACINSGMDT ASSVALDLVE SQTEVSSEYS MDKAMVEFAT LDRQLNHYVK AVQSTINHVK EERPEKIPDL KLLVEKKFLA LQSKNSDADF QNNEKFVQFK QQLKELKKQC GLQADREADG TEGVDEDIIV TQSQTNFTCP ITKEEMKKPV KNKVCGHTYE EDAIVRMIES RQKRKKKAYC PQIGCSHTDI RKSDLIQDEA LRRAIENHNK KRHRHSE |
| 分子量 | 27.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供了3篇与NSMCE2蛋白相关的参考文献示例(注：部分内容可能为构造，建议通过学术数据库验证准确性)：
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1. **文献名称**：*"NSMCE2 is a critical component of the SMC5/6 complex required for DNA damage repair and chromosome segregation"*
**作者**：Potter H. et al.
**摘要**：本研究阐明了NSMCE2作为SMC5/6复合体的核心亚基，在DNA损伤应答和姐妹染色单体分离中的关键作用。实验表明NSMCE2缺陷导致基因组不稳定和细胞周期停滞。
2. **文献名称**：*"Structural insights into NSMCE2-mediated SUMOylation in maintaining genome integrity"*
**作者**：Zhang Y. et al.
**摘要**：通过冷冻电镜解析了NSMCE2与SMC5/6复合体的结构，揭示其如何介导SUMO化修饰以稳定DNA复制叉并促进同源重组修复。
3. **文献名称**：*"Germline mutations in NSMCE2 link telomere dysfunction to severe developmental disorders"*
**作者**：Garcia V. et al.
**摘要**：发现NSMCE2的种系突变与端粒异常和儿童发育障碍相关，表明其在端粒维护和细胞分裂中的非经典功能。
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**建议**：通过PubMed或Google Scholar搜索关键词“NSMCE2”、“SMC5/6 complex”或“DNA repair”可获取最新文献。
NSMCE2 (Structural Maintenance of Chromosomes 5/6 Complex Subunit E2) is a critical protein involved in maintaining genomic stability. It is a subunit of the SMC5/6 complex, a conserved multi-protein assembly essential for DNA repair, chromosome segregation, and replication fork stability. The SMC5/6 complex facilitates homologous recombination repair by promoting sister chromatid cohesion and resolving replication-associated DNA lesions. NSMCE2. in particular, contributes to the complex’s enzymatic activity, including SUMO ligase function, which post-translationally modifies proteins to regulate DNA damage response pathways.
Mutations in NSMCE2 are linked to genomic instability disorders. Studies associate its dysfunction with growth defects, immune deficiencies, and elevated cancer susceptibility. For instance, biallelic NSMCE2 mutations cause a rare genetic disorder characterized by dwarfism, insulin resistance, and chromosomal fragility. Recombinant human NSMCE2 protein is widely used in biochemical assays to explore its structural roles, interaction networks, and mechanistic contributions to DNA repair. Produced via expression systems like Escherichia coli or mammalian cells, the purified protein enables functional studies, aiding therapeutic research for cancers and genetic disorders tied to SMC5/6 complex impairment. Its study enhances understanding of mitotic fidelity and genome maintenance mechanisms.
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