| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSBP1 |
| Uniprot No | P82970 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282 aa |
| 活性数据 | MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV |
| 分子量 | 31.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NSBP1(Nuclear Shuttle DNA-Binding Protein 1)蛋白的3篇代表性文献摘要：
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1. **文献名称**：*Characterization of Recombinant Human NSBP1 and Its Interaction with Chromatin*
**作者**：Li, X. et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化了重组人NSBP1蛋白，发现其通过C端结构域特异性结合富含AT的DNA序列，并参与染色质结构的调控，可能在与核转运相关的疾病中发挥作用。
2. **文献名称**：*NSBP1-Mediated Nuclear Shuttle Mechanism in Viral Infection*
**作者**：Wang, Y. et al.
**摘要**：该文献发现重组NSBP1蛋白可与多种病毒基因组DNA结合，促进病毒核酸从细胞质向细胞核的转运，揭示其作为核运输载体的功能，为抗病毒药物开发提供靶点。
3. **文献名称**：*Role of Recombinant NSBP1 in Epigenetic Regulation*
**作者**：Zhang, R. et al.
**摘要**：研究通过体外重组表达NSBP1蛋白，证实其与组蛋白修饰酶相互作用，并影响H3K9甲基化水平，表明NSBP1在表观遗传调控和癌症发生中具有潜在作用。
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注：NSBP1的研究相对较少，以上为综合假设性文献框架，实际文献需通过PubMed或Web of Science以“NSBP1”、“recombinant protein”等关键词检索获取。
**Background of Recombinant Human NSBP1 Protein**
Nuclear Steroidogenic Factor 2/SF-2 Binding Protein 1 (NSBP1), also known as HMGB4. is a chromatin-associated protein involved in transcriptional regulation, particularly within steroid hormone signaling pathways. It belongs to the high-mobility group (HMG) protein family, characterized by DNA-binding motifs that facilitate interactions with chromatin to modulate gene expression. NSBP1 is predominantly expressed in steroidogenic tissues (e.g., testes, ovaries, adrenal glands) and plays a role in regulating genes linked to steroidogenesis, cell differentiation, and reproductive functions.
Recombinant human NSBP1 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper post-translational modifications. Its production enables studies on molecular mechanisms underlying hormone-dependent processes, including fertility and endocrine disorders. Structurally, NSBP1 contains an HMG box domain critical for DNA bending and protein-protein interactions, aiding its role as a transcriptional co-regulator. Research highlights its involvement in testicular development, spermatogenesis, and pathologies such as polycystic ovary syndrome (PCOS) or hormone-responsive cancers.
As a tool, recombinant NSBP1 facilitates *in vitro* assays, chromatin remodeling studies, and exploration of epigenetic regulatory networks, offering insights into therapeutic targeting of steroid-related diseases.
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