| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NS4ATP2 |
| Uniprot No | Q9HAJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183 aa |
| 活性数据 | MNGFSTEEDS REGPPAAPAA AAPGYGQSCC LIEDGERCVR PAGNASFSKR VQKSISQKKL KLDIDKSVRH LYICDFHKNF IQSVRNKRKR KTSDDGGDSP EHDTDIPEVD LFQLQVNTLR RYKRHYKLQT RPGFNKAQLA ETVSRHFRNI PVNEKETLAY FIYMVKSNKS RLDQKSEGGK QLE |
| 分子量 | 20.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NS4ATP2蛋白的虚构参考文献示例(因实际文献可能有限或名称存在差异，建议核实名称准确性)：
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1. **文献名称**：*Functional Characterization of Recombinant Human NS4ATP2 in Cell Cycle Regulation*
**作者**：Zhang L. et al.
**摘要**：本研究成功表达并纯化了重组人NS4ATP2蛋白，证实其通过与细胞周期蛋白复合物相互作用调控G1/S期转换，可能为癌症治疗提供靶点。
2. **文献名称**：*NS4ATP2 Interacts with Viral Proteins: Implications for Host-Pathogen Interactions*
**作者**：Chen W. et al.
**摘要**：利用重组NS4ATP2蛋白进行体外结合实验，发现其与多种病毒蛋白(如HCV NS4A)结合，提示其在宿主抗病毒反应中的潜在作用。
3. **文献名称**：*Optimization of Recombinant NS4ATP2 Expression in E. coli for Structural Studies*
**作者**：Kimura T. et al.
**摘要**：报道了一种高效原核表达系统生产可溶性NS4ATP2蛋白，并通过X射线晶体学解析其三维结构，揭示了ATP结合域的关键位点。
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**注意**：以上为示例文献，实际研究中建议通过 **PubMed** 或 **Google Scholar** 输入关键词(如"NS4ATP2"、"recombinant protein")检索最新成果。若名称无误且文献极少，可拓展搜索相关基因家族(如ATP酶家族)或功能(如病毒宿主互作)。
Recombinant human NS4ATP2 (non-structural protein 4 ATPase 2) is a genetically engineered protein derived from the human NS4ATP2 gene. This protein belongs to the AAA+ ATPase family, characterized by conserved ATP-binding motifs critical for energy-dependent processes such as protein folding, membrane trafficking, and nucleic acid remodeling. NS4ATP2 is implicated in cellular stress responses, DNA repair mechanisms, and mitochondrial function, though its exact biological pathways remain under investigation. Studies suggest it may interact with viral replication complexes, potentially serving as a host factor in viral pathogenesis, which has spurred interest in antiviral therapy research.
Produced via recombinant DNA technology, NS4ATP2 is typically expressed in bacterial (e.g., E. coli) or mammalian cell systems, followed by purification using affinity chromatography. The recombinant form retains the conserved Walker A/B motifs and helicase domains essential for ATP hydrolysis and substrate binding. Current research focuses on its structural dynamics through cryo-EM and X-ray crystallography, revealing conformational changes during ATPase cycling. Pharmaceutical applications explore NS4ATP2 as a potential biomarker for cellular stress disorders and a therapeutic target for viral infections, though clinical relevance requires further validation. Its dual role in cellular homeostasis and pathogen interactions makes it a compelling subject for interdisciplinary studies.
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