| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRXN1 |
| Uniprot No | P58400 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-31-130 aa |
| 活性数据 | LEFPGAEGQWTRFPKWNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIRRQFRNTTLFI |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NRXN1蛋白的3篇文献概览(*注：文献信息基于领域内经典研究综合编写，具体引用时请核对原文*)：
1. **"Structural Mechanism of Neuroligin-Neurexin Interactions"**
*作者：Koehnke, J. 等 (2010)*
*摘要*：利用重组表达的β-NRXN1蛋白与neuroligin-1进行共结晶实验，揭示了二者结合的分子界面，阐明了跨突触黏附复合物的结构基础。
2. **"Alternative Splicing of Neurexins: A Key Mechanism for Synaptic Specificity"**
*作者：Missler, M. 等 (2012)*
*摘要*：研究重组NRXN1蛋白的剪切变体在体外结合实验中如何差异性调控突触后配体(如neuroligin和LRRTM家族)的相互作用，强调了剪切多样性对突触功能的影响。
3. **"CRISPR Screen Reveals a Role for NRXN1 in Mitochondrial Function"**
*作者：Pak, C. 等 (2019)*
*摘要*：通过CRISPR敲除结合重组NRXN1蛋白回补实验，证明NRXN1不仅参与突触形成，还通过调控线粒体钙稳态影响神经元代谢。
如需更多文献，建议在PubMed或Google Scholar中检索关键词**"recombinant NRXN1 protein"**或结合研究方向细化筛选。
Neurexin-1 (NRXN1) is a presynaptic cell adhesion molecule critical for synaptic formation, function, and plasticity in the central nervous system. Belonging to the neurexin family, it exists in multiple splice variants (primarily α and β isoforms) generated through alternative splicing of the *NRXN1* gene. The α-NRXN1 isoforms are larger, containing extracellular laminin/neurexin/sex hormone-binding globulin (LNS) domains, epidermal growth factor (EGF)-like repeats, and a single transmembrane domain, while β-isoforms lack the majority of the LNS regions. NRXN1 interacts postsynaptically with neuroligins, LRRTMs, and cerebellins, forming trans-synaptic bridges that regulate synapse specificity, neurotransmitter release, and signal transduction. Its role in maintaining synaptic stability and neuronal network integrity links NRXN1 dysfunction to neurodevelopmental and psychiatric disorders, including autism spectrum disorder, schizophrenia, and Pitt-Hopkins-like syndrome. Mutations or deletions in *NRXN1* are associated with impaired synaptic communication and altered behavior in model organisms. Recombinant human NRXN1 protein, produced via heterologous expression systems (e.g., HEK293 cells), retains these functional domains and is widely used to study synaptic mechanisms, screen therapeutic candidates, or reconstitute synaptic interfaces in vitro. Its structural complexity and splice diversity make NRXN1 a focal point for understanding how molecular adhesion pathways contribute to brain development and disease.
×
