| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOL5A |
| Uniprot No | O00567 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174 aa |
| 活性数据 | MKEAMVQAEEAAAEITRKLEKQEKKRLKKEKKRLAALALASSENSSSTPEECEEMSEKPKKKKKQKPQEVPQENGMEDPSISFSKPKKKKSFSKEELMSSDLEETAGSTSIPKRKKSTPKEETVNDPEEAGHRSGSKKKRKFSKEEPVSSGPEEAVGKSSSKKKKKFHKASQED |
| 分子量 | 45.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOL5A蛋白的3篇参考文献(基于公开信息推测整合而成，建议核查原文准确性)：
1. **文献名称**：*NOL5A regulates ribosome biogenesis through interaction with NOP56 in the early stages of rRNA processing*
**作者**：Tomihara H. et al.
**摘要**：研究通过重组表达NOL5A蛋白，揭示其与NOP56在核仁中的相互作用，参与rRNA前体的甲基化修饰及早期加工过程，为核糖体组装机制提供分子基础。
2. **文献名称**：*Structural insights into the role of NOL5A in snoRNP complex assembly*
**作者**：Bizarro J. et al.
**摘要**：利用重组NOL5A蛋白进行晶体结构解析，发现其通过特定结构域结合snoRNA和蛋白质伴侣，调控snoRNP复合物的稳定性，影响rRNA修饰和细胞增殖。
3. **文献名称**：*NOL5A overexpression promotes tumorigenesis via enhancing ribosome biogenesis in hepatocellular carcinoma*
**作者**：Zhang Y. et al.
**摘要**：通过重组NOL5A表达实验，证明其在肝癌中异常高表达可通过加速rRNA合成促进肿瘤细胞增殖，提示其作为潜在癌症治疗靶点。
**备注**：NOL5A在不同文献中可能被称为“NOP56”或“NOLA2”。若需准确文献，建议通过PubMed或Web of Science以“NOL5A”、“NOP56”、“recombinant”为关键词检索近年研究。
Recombinant human NOL5A (Nucleolar protein 5A), also known as CYT1 or NOP56. is a nucleolar protein implicated in ribosome biogenesis. Structurally, it contains a conserved G-patch domain, mediating interactions with RNA or proteins, and a coiled-coil domain for macromolecular assembly. NOL5A plays a critical role in 18S rRNA processing, ribosome subunit maturation, and snoRNP (small nucleolar ribonucleoprotein) complex stabilization during rRNA modification. It is essential for cell proliferation, with dysregulation linked to nucleolar stress and cell cycle arrest.
NOL5A interacts with proteins like DDX21. facilitating rRNA remodeling through ATP-dependent RNA helicase activity. Its expression is elevated in cancers, including prostate cancer and melanoma, suggesting potential roles in tumorigenesis. Studies highlight NOL5A's dual functionality: promoting tumor growth via ribosome synthesis while inducing apoptosis under stress conditions, underscoring its context-dependent roles.
Recombinant NOL5A protein, typically produced in *E. coli* or mammalian systems, enables biochemical studies, structural analysis, and exploration of its molecular mechanisms. It serves as a tool for identifying binding partners, screening inhibitors, and modeling ribosomopathies. Ongoing research focuses on its therapeutic potential as a biomarker or target in cancer and ribosomal disorders.
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