关键信息
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基因名
PPP3CC
- 应用
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别名
PPP3CC；CALNA3；CNA3；Serine/threonine-Protein phosphatase 2B catalytic subunit gamma isoform
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48454
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表达区间
2-512aa
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氨基酸序列
SGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQEKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS
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分子量
62.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PPP3CC, also known as protein phosphatase 3, catalytic subunit gamma, plays a crucial role in various cellular processes by dephosphorylating serine and threonine residues on target proteins. It is a component of the protein phosphatase 2B (PP2B) complex, which is essential for calcium/calmodulin-dependent signaling pathways. Dysregulation of PPP3CC has been implicated in several diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders. Research has focused on understanding its structure, function, and regulatory mechanisms to elucidate its involvement in pathological conditions and identify potential therapeutic targets. The recombinant expression of PPP3CC enables scientists to study its enzymatic activity, interaction with different substrates, and effects of various inhibitors. This research provides insights into the fundamental biology of cellular signaling and reveals the potential of PPP3CC as a biomarker or therapeutic target in disease contexts, promoting the advancement of novel treatment strategies. By investigating the precise role of PPP3CC in cellular signaling pathways, researchers aim to develop a deeper understanding of its contributions to health and disease.
