| WB | 1:500-2000 | human |
| FCM | 1:50-200 | human |
12 months from date of receipt，-20℃ as supplied.
Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody . The sample well of each lane was loaded with 30 ug of sample
under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HELA whole cell lysates,
Lane 3: human A549 whole cell lysates.
After
electrophoresis, proteins were transferred to a membrane. Then the
membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity
purified polyclonal antibody at a dilution of 1:1000 and
probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting
Substrate . A specific band was detected for
MKP-1/DUSP1 at approximately 40 kDa. The expected band size for
MKP-1/DUSP1 is at 39 kDa.
Flow Cytometry analysis of Caco-2 cells using anti-MKP-1/DUSP1 antibody .
Overlay
histogram showing Caco-2 cells stained with (Blue line). To
facilitate intracellular staining, cells were fixed with 4%
paraformaldehyde and permeabilized with permeabilization buffer. The
cells were blocked with 10% normal goat serum. And then incubated with
rabbit anti-MKP-1/DUSP1 Antibody at 1:100 dilution for 30 min
at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used
as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype
control antibody (Green line) was rabbit IgG at 1:100 dilution used
under the same conditions. Unlabelled sample without incubation with
primary antibody and secondary antibody (Red line) was used as a blank
control.
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