| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL43 |
| Uniprot No | Q8N983 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159 aa |
| 活性数据 | MTARGTPSRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNSRPCCVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKPTTFRGLRPREVQDPAPAQVQAQ |
| 分子量 | 44.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPL43蛋白的3篇参考文献概述：
---
1. **"Mitochondrial ribosomal protein L43 regulates cell cycle progression in human cancer cells"**
*作者：Zhang Y, et al.*
摘要：研究揭示了MRPL43通过调控线粒体翻译影响细胞周期蛋白表达，其重组蛋白被用于体外功能实验，表明敲低MRPL43会导致G1期阻滞并抑制肿瘤生长。
---
2. **"Structural insights into the assembly mechanism of the mitochondrial ribosome: the role of MRPL43"**
*作者：Wang L, et al.*
摘要：通过重组表达MRPL43蛋白并结合冷冻电镜技术，阐明了该蛋白在线粒体核糖体大亚基组装中的关键作用，提出了其与RNA结合域的结构互作模型。
---
3. **"Functional characterization of MRPL43 mutations associated with mitochondrial encephalopathy"**
*作者：Smith J, et al.*
摘要：利用重组MRPL43蛋白进行突变体功能回补实验，发现其某些突变会破坏线粒体呼吸链复合物活性，导致ATP合成缺陷，与神经退行性疾病相关。
---
注：以上文献为模拟概括，实际研究需根据具体实验目标检索PubMed等数据库获取。
**Background of Recombinant Human MRPL43 Protein**
Mitochondrial ribosomal protein L43 (MRPL43) is a nuclear-encoded component of the large subunit (39S) of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, responsible for cellular energy production via oxidative phosphorylation, rely on their own translational machinery to synthesize subunits of the electron transport chain complexes. MRPL43 plays a critical role in ribosome assembly/stability and ensures fidelity during mitochondrial mRNA translation. Dysregulation of mitochondrial ribosome proteins, including MRPL43. has been linked to mitochondrial dysfunction, metabolic disorders, and cancers.
Recombinant MRPL43 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Its recombinant form allows researchers to investigate molecular mechanisms of mitochondrial translation, interactions with ribosomal subunits or cofactors, and potential roles in diseases. Structural analysis of MRPL43. often combined with cryo-EM or X-ray crystallography, provides insights into ribosome architecture. Additionally, recombinant MRPL43 serves as an antigen for antibody development or a tool to model pathogenic mutations associated with tissue-specific mitochondrial deficiencies. Current research focuses on elucidating its role in cellular metabolism and therapeutic targeting of mitochondrial disorders.
×
