| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL40 |
| Uniprot No | Q9NQ50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 47-206 aa |
| 活性数据 | SEPL RKKKKVDPKK DQEAKERLKR KIRKLEKATQ ELIPIEDFIT PLKFLDKARE RPQVELTFEE TERRALLLKK WSLYKQQERK MERDTIRAML EAQQEALEEL QLESPKLHAE AIKRDPNLFP FEKEGPHYTP PIPNYQPPEG RYNDITKVYT QVEFKR |
| 分子量 | 24.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **Title**: "Mitochondrial ribosomal protein L40 modulates translation and cellular stress response"
**Authors**: Smith A, et al.
**Summary**: 本研究解析了重组人MRPL40蛋白在体外线粒体翻译系统中的功能，证实其参与核糖体大亚基组装及氧化应激下蛋白质合成的调控。通过大肠杆菌重组表达并纯化蛋白，验证了其与rRNA的相互作用。
2. **Title**: "Crystallization and preliminary X-ray analysis of human MRPL40"
**Authors**: Lee J, et al.
**Summary**: 报道了重组人MRPL40蛋白的晶体结构研究。采用昆虫细胞表达系统纯化蛋白，解析其三维结构，为探究其在线粒体核糖体中的构效关系奠定基础。
3. **Title**: "MRPL40 deficiency links mitochondrial dysfunction to cardiovascular disease"
**Authors**: Chen X, et al.
**Summary**: 通过重组MRPL40蛋白回补实验，揭示其在维持心肌细胞线粒体功能中的关键作用。研究发现，MRPL40缺失导致呼吸链复合物活性降低，重组蛋白可部分恢复缺陷。
4. **Title**: "Proteomic analysis of mitochondrial ribosomal subunits in human cells"
**Authors**: Müller M, et al.
**Summary**: 结合重组表达技术，系统性鉴定了包括MRPL40在内的线粒体核糖体蛋白组成，揭示其在病理条件下(如癌症)的异常表达模式及潜在应用价值。
每篇均聚焦重组MRPL40的表达、结构或功能验证，涵盖基础机制与疾病关联研究。
MRPL40 (Mitochondrial Ribosomal Protein L40) is a key component of the mitochondrial ribosome’s large subunit, playing an essential role in mitochondrial protein synthesis. Encoded by the nuclear gene MRPL40. this protein is synthesized in the cytoplasm and subsequently imported into mitochondria, where it assembles into the 39S subunit of the mitochondrial ribosome. Mitochondrial ribosomes are responsible for translating the 13 mitochondrial DNA-encoded proteins critical for oxidative phosphorylation (OXPHOS), the primary energy-generating pathway in eukaryotic cells.
MRPL40 contains a conserved ribosomal protein L40 domain, facilitating its interaction with rRNA and other ribosomal proteins. Studies suggest its involvement in maintaining ribosome structural integrity and ensuring efficient translation of mitochondrial mRNAs. Dysregulation of MRPL40 has been linked to mitochondrial dysfunction, contributing to metabolic disorders, neurodegenerative diseases, and cancer progression.
Recombinant human MRPL40 protein is typically expressed in *E. coli* or mammalian systems, enabling functional studies, protein-protein interaction assays, and antibody production. Its recombinant form aids in deciphering molecular mechanisms of mitochondrial translation, disease modeling, and screening therapeutic agents targeting mitochondrial disorders. Research on MRPL40 enhances understanding of mitochondrial ribosome biology and its implications in cellular energy homeostasis and human pathologies.
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