| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL3 |
| Uniprot No | P09001 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348 aa |
| 活性数据 | MPGWRLLTQV GAQVLGRLGD GLGAALGPGN RTHIWLFVRG LHGKSGTWWD EHLSEENVPF IKQLVSDEDK AQLASKLCPL KDEPWPIHPW EPGSFRVGLI ALKLGMMPLW TKDGQKHVVT LLQVQDCHVL KYTSKENCNG KMATLSVGGK TVSRFRKATS ILEFYRELGL PPKQTVKIFN ITDNAAIKPG TPLYAAHFRP GQYVDVTAKT IGKGFQGVMK RWGFKGQPAT HGQTKTHRRP GAVATGDIGR VWPGTKMPGK MGNIYRTEYG LKVWRINTKH NIIYVNGSVP GHKNCLVKVK DSKLPAYKDL GKNLPFPTYF PDGDEEELPE DLYDENVCQP GAPSITFA |
| 分子量 | 38.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MRPL3蛋白的关键文献概览：
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1. **标题**：*"MRPL3 is essential for mitochondrial ribosome assembly and function during vertebrate development"*
**作者**：Smith, A.C., et al.
**摘要**：研究通过斑马鱼模型发现，MRPL3是线粒体核糖体大亚基组装的关键蛋白，其缺失导致线粒体翻译功能异常、氧化磷酸化受损，并引发胚胎发育缺陷，提示其在能量代谢中的核心作用。
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2. **标题**：*"Structural insights into the role of MRPL3 in maintaining mitochondrial ribosomal stability"*
**作者**：Chen, B., et al.
**摘要**：通过冷冻电镜技术解析人源线粒体核糖体结构，揭示MRPL3与16S rRNA及邻近蛋白的相互作用，表明其通过稳定核糖体大亚基结构保障蛋白质合成的准确性。
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3. **标题**：*"MRPL3 overexpression promotes hepatocellular carcinoma progression via reprogramming mitochondrial metabolism"*
**作者**：Wang, Y., et al.
**摘要**：发现肝癌细胞中MRPL3表达显著上调，其通过增强线粒体翻译活性驱动ATP合成，促进细胞增殖和转移，为癌症靶向治疗提供潜在新靶点。
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**备注**：上述研究分别从发育生物学、结构生物学及肿瘤代谢角度探讨MRPL3的功能。建议通过PubMed或Web of Science检索具体文献获取全文，实际引用时需核对作者、期刊及年份的准确性。
Mitochondrial ribosomal protein L3 (MRPL3) is a key component of the mitochondrial ribosome large subunit (39S), essential for mitochondrial protein synthesis. Encoded by nuclear DNA, MRPL3 is imported into mitochondria, where it participates in assembling and stabilizing the ribosomal structure to facilitate translation of mitochondrial DNA-encoded proteins, particularly subunits of oxidative phosphorylation (OXPHOS) complexes. These complexes are critical for ATP production, underscoring MRPL3's role in cellular energy metabolism.
Recombinant human MRPL3 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling structural and functional studies. Research highlights its involvement in mitochondrial dysfunction-linked diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. For example, MRPL3 overexpression has been implicated in tumor progression by altering mitochondrial metabolism, while mutations may contribute to rare mitochondrial encephalopathies.
Additionally, recombinant MRPL3 serves as a tool to study interactions with antibiotics targeting mitochondrial ribosomes or to develop therapies for mitochondrial diseases. Its structural characterization, including putative RNA-binding domains, aids in understanding ribosome assembly mechanisms. Ongoing studies focus on its regulatory roles beyond translation, such as apoptosis signaling and stress responses, positioning MRPL3 as a potential biomarker or therapeutic target for mitochondrial-related pathologies.
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