| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPHOSPH8 |
| Uniprot No | Q99549 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259 aa |
| 活性数据 | MTLVMLAAAGGQDDLLRLLITKGAKVNGRQKNGTTALIHAAEKNFLTTVAILLEAGAFVNVQQSNGETALMKACKRGNSDIVRLVIECGADCNILSKHQNSALHFAKQSNNVLVYDLLKNHLETLSRVAEETIKDYFEARLALLEPVFPIACHRLCEGPDFSTDFNYKPPQNIPEGSGILLFIFHANFLGKEVIARLCGPCSVQAVVLNDKFQLPVFLDSHFVYSFSPVAGPNKLFIRLTEAPSAKVKLLIGAYRVQLQ |
| 分子量 | 54.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人MPHOSPH8(M-phase phosphoprotein 8)蛋白相关的模拟参考文献示例，根据该蛋白已知生物学背景归纳整理：
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1. **文献名称**: *"MPHOSPH8 regulates mitotic progression through interaction with Aurora kinase B"*
**作者**: Chen L, et al.
**摘要**: 本研究在大肠杆菌系统中表达了重组人MPHOSPH8蛋白，并验证其在有丝分裂中的作用。通过共免疫沉淀实验证明MPHOSPH8直接与Aurora B激酶互作，调控纺锤体组装检查点功能，其磷酸化修饰是细胞周期G2/M转换的关键调控点。
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2. **文献名称**: *"Structural insights into the interaction of MPHOSPH8 with the anaphase-promoting complex"*
**作者**: Yamamoto K, et al.
**摘要**: 通过X射线晶体学解析了重组MPHOSPH8蛋白与后期促进复合物(APC/C)的Cdh1亚基结合的结构，揭示了其C端螺旋结构域对APC/C活性的调控机制，为癌症治疗中细胞周期靶点提供了新思路。
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3. **文献名称**: *"MPHOSPH8 overexpression promotes chromosomal instability in hepatocellular carcinoma"*
**作者**: Wang Q, et al.
**摘要**: 研究利用His标签重组MPHOSPH8蛋白进行体外功能实验，发现其在肝癌细胞中异常高表达导致染色体分离错误和微核形成，敲低后显著抑制肿瘤增殖。结合临床数据分析提示MPHOSPH8可作为肝癌预后标志物。
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**说明**：以上文献为基于MPHOSPH8已知功能(细胞周期调控、癌症关联)的模拟案例，实际研究需以PubMed/SCI数据库检索结果为准。建议通过关键词“MPHOSPH8 OR Mphosph8 AND recombinant”查询最新文献，如涉及具体应用(疾病机制/结构解析等)，可进一步限定研究方向。
**Background of Recombinant Human MPHOSPH8 Protein**
The MPHOSPH8 (M-phase phosphoprotein 8) protein, also termed MPP8. is a component of the human chromosomal passenger complex (CPC), which plays a pivotal role in regulating mitosis and chromosome segregation. This 835-amino-acid protein contains conserved domains, including a coiled-coil region and a C-terminal chromo shadow domain, facilitating interactions with histones and other regulatory proteins. MPHOSPH8 is phosphorylated during the G2/M phase transition, contributing to spindle assembly, kinetochore-microtubule attachment, and cytokinesis.
Dysregulation of MPHOSPH8 is linked to mitotic errors, genomic instability, and cancer progression. Overexpression has been observed in malignancies such as hepatocellular carcinoma and glioblastoma, correlating with poor prognosis. Its interaction with Aurora kinases and PLK1 underscores its role in checkpoint signaling and cell cycle control.
Recombinant MPHOSPH8 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purified through affinity chromatography (e.g., His-tag), it serves as a tool to investigate mitotic mechanisms, screen anticancer drugs, or explore epigenetic regulation via its association with HP1 proteins and heterochromatin. Current research focuses on targeting MPHOSPH8 to mitigate tumorigenesis and chemoresistance.
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