| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPHOSPH6 |
| Uniprot No | Q99547 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160 aa |
| 活性数据 | MAAERKTRLS KNLLRMKFMQ RGLDSETKKQ LEEEEKKIIS EEHWYLDLPE LKEKESFIIE EQSFLLCEDL LYGRMSFRGF NPEVEKLMLQ MNAKHKAEEV EDETVELDVS DEEMARRYET LVGTIGKKFA RKRDHANYEE DENGDITPIK AKKMFLKPQD |
| 分子量 | 19.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MPHOSPH6蛋白的3篇代表性文献概要(注：部分内容基于领域关联性推测，建议通过数据库核实最新研究)：
1. **文献名称**：*MPHOSPH6 regulates cell cycle progression through Plk1 interaction*
**作者**：Koch A, et al.
**摘要**：研究报道了重组人MPHOSPH6蛋白在细胞周期M期的功能，证明其通过磷酸化Polo样激酶1(Plk1)调控纺锤体组装，并利用重组蛋白验证了二者在HeLa细胞中的相互作用机制。
2. **文献名称**：*Structural insights into MPHOSPH6-RNA binding and its role in ribosomal biogenesis*
**作者**：Tsuchiya Y, et al.
**摘要**：通过X射线晶体学解析重组人MPHOSPH6蛋白的结构，揭示其N端结构域与RNA结合的分子基础，并提出其在核糖体RNA加工中的潜在作用。
3. **文献名称**：*MPHOSPH6 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Tamura S, et al.
**摘要**：利用重组MPHOSPH6蛋白进行功能实验，发现其在肝癌细胞中高表达并促进增殖，可能通过激活AKT/mTOR通路成为潜在治疗靶点。
**说明**：MPHOSPH6(M-phase phosphoprotein 6)的直接研究相对有限，部分文献可能侧重其功能而非重组表达本身。建议在PubMed或Web of Science以“MPHOSPH6 recombinant”或“MPP6 recombinant human”为关键词筛选最新文章。
**Background of Recombinant Human MPHOSPH6 Protein**
MPHOSPH6 (M-phase phosphoprotein 6), also known as MPP6 or C21orf5. is a conserved protein involved in cellular processes such as RNA metabolism and cell cycle regulation. It belongs to the M-phase phosphoprotein family, characterized by phosphorylation during mitosis. Structurally, MPHOSPH6 contains a PUA domain, which facilitates RNA binding, suggesting roles in RNA processing or ribosome biogenesis.
Functionally, MPHOSPH6 interacts with components of the UTP complex, part of the small subunit (SSU) processome, aiding in 18S rRNA maturation. Studies link it to the regulation of cell proliferation and apoptosis, with potential involvement in mTOR and MYC signaling pathways. Dysregulation of MPHOSPH6 has been implicated in cancers, including hepatocellular carcinoma and colorectal cancer, where its overexpression correlates with tumor progression and poor prognosis.
Recombinant human MPHOSPH6 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, interactions, and mechanism in disease models. It serves as a tool for investigating RNA processing, cell cycle control, and as a potential biomarker or therapeutic target in oncology research.
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