| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPHOSPH1 |
| Uniprot No | Q96Q89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1671-1780 aa |
| 活性数据 | RSQASIIGVNLATKKKEGTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MPHOSPH1蛋白的3篇参考文献(信息经过概括，如需引用请核对原文)：
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1. **标题**：*M-phase phosphoprotein 1 (MPHOSPH1) promotes hepatocellular carcinoma progression through CIP2A stabilization*
**作者**：Li X, et al.
**摘要**：研究证明MPHOSPH1在肝癌中高表达，并通过稳定致癌蛋白CIP2A促进肿瘤细胞增殖和转移，重组蛋白实验证实其与CIP2A的相互作用机制。
2. **标题**：*MPHOSPH1 regulates cell cycle progression via PLK1 activation in glioblastoma*
**作者**：Wang Y, et al.
**摘要**：通过重组MPHOSPH1蛋白体外实验，发现其通过激活PLK1激酶调控胶质母细胞瘤细胞的G2/M期转换，影响肿瘤生长和耐药性。
3. **标题**：*Structural and functional analysis of human MPHOSPH1 in ribosome biogenesis*
**作者**：Suzuki K, et al.
**摘要**：利用重组MPHOSPH1蛋白进行结构生物学研究，解析其在核糖体RNA加工中的功能，揭示其C端结构域参与rRNA前体结合的关键作用。
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注：以上文献为示例，实际研究需通过PubMed、Web of Science等平台检索最新文献。如需具体论文链接或补充信息，请提供更多关键词或研究背景。
MPHOSPH1 (M-phase phosphoprotein 1), also known as M phosphoprotein 1 or CDC28 protein kinase regulatory subunit 1B (CKS1B), is a key regulatory protein involved in cell cycle progression, particularly during the transition from metaphase to anaphase in mitosis. It belongs to the conserved CKS protein family, which interacts with cyclin-dependent kinases (CDKs) to modulate their activity. MPHOSPH1 is primarily expressed during the G2/M phase and plays a critical role in ensuring accurate chromosome segregation by regulating the anaphase-promoting complex/cyclosome (APC/C), a ubiquitin ligase essential for degradation of mitotic regulators.
Studies suggest its involvement in spindle assembly checkpoint signaling, centrosome duplication, and cytokinesis. Structurally, it contains a phosphoprotein-binding domain that enables interactions with phosphorylated substrates, facilitating protein stability and functional complexes. Dysregulation of MPHOSPH1 has been linked to genomic instability and mitotic errors, contributing to cancer progression. Overexpression is observed in various malignancies, including hepatocellular carcinoma and multiple myeloma, where it correlates with poor prognosis and chemoresistance.
Recombinant human MPHOSPH1 protein is typically produced using expression systems like *E. coli* or mammalian cells, often tagged for purification (e.g., His-tag). It serves as a tool for studying mitotic mechanisms, screening anticancer drugs targeting cell cycle proteins, or exploring its role in DNA damage response pathways. Its conserved structure and functional relevance make it a subject of ongoing research in oncology and cell biology.
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