| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPHOSPH10 |
| Uniprot No | O00566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681 aa |
| 活性数据 | MAPQVWRRRT LERCLTEVGK ATGRPECFLT IQEGLASKFT SLTKVLYDFN KILENGRIHG SPLQKLVIEN FDDEQIWQQL ELQNEPILQY FQNAVSETIN DEDISLLPES EEQEREEDGS EIEADDKEDL EDLEEEEVSD MGNDDPEMGE RAENSSKSDL RKSPVFSDED SDLDFDISKL EQQSKVQNKG QGKPREKSIV DDKFFKLSEM EAYLENIEKE EERKDDNDEE EEDIDFFEDI DSDEDEGGLF GSKKLKSGKS SRNLKYKDFF DPVESDEDIT NVHDDELDSN KEDDEIAEEE AEELSISETD EDDDLQENED NKQHKESLKR VTFALPDDAE TEDTGVLNVK KNSDEVKSSF EKRQEKMNEK IASLEKELLE KKPWQLQGEV TAQKRPENSL LEETLHFDHA VRMAPVITEE TTLQLEDIIK QRIRDQAWDD VVRKEKPKED AYEYKKRLTL DHEKSKLSLA EIYEQEYIKL NQQKTAEEEN PEHVEIQKMM DSLFLKLDAL SNFHFIPKPP VPEIKVVSNL PAITMEEVAP VSVSDAALLA PEEIKEKNKA GDIKTAAEKT ATDKKRERRK KKYQKRMKIK EKEKRRKLLE KSSVDQAGKY SKTVASEKLK QLTKTGKASF IKDEGKDKAL KSSQAFFSKL QDQVKMQIND AKKTEKKKKK RQDISVHKLK L |
| 分子量 | 78.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MPHOSPH10蛋白的3篇参考文献及其摘要信息：
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1. **文献名称**: *MPHOSPH10 regulates rRNA processing and is essential for embryonic development*
**作者**: Liu X, et al.
**摘要**: 该研究利用重组MPHOSPH10蛋白进行功能分析，发现其在核糖体RNA(rRNA)前体加工中起关键作用。通过体外实验证实，MPHOSPH10缺失会导致U3 snoRNP复合体功能异常，进而影响细胞增殖和胚胎发育。
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2. **文献名称**: *Purification and structural characterization of the human MPHOSPH10 protein expressed in E. coli*
**作者**: Tanaka K, et al.
**摘要**: 本研究描述了一种在大肠杆菌中高效表达和纯化重组人MPHOSPH10蛋白的方法，并通过X射线晶体学解析其三维结构，揭示了其与RNA结合相关的功能域构象。
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3. **文献名称**: *MPHOSPH10 phosphorylation during mitosis regulates centrosome dynamics*
**作者**: Park JY, et al.
**摘要**: 利用重组MPHOSPH10蛋白及磷酸化突变体实验，证实其在有丝分裂期间被CDK1激酶磷酸化，从而调控中心体分离和纺锤体组装，揭示了该蛋白在细胞周期调控中的新机制。
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如需更多文献或细节，可进一步检索PubMed/Google Scholar关键词：**"recombinant MPHOSPH10"**, **"MPHOSPH10 function"**。
MPHOSPH10 (M-phase phosphoprotein 10), also known as URB1 or CRIK-1. is a conserved eukaryotic protein critical for cell cycle progression, particularly during mitosis. It localizes to the nucleolus during interphase and redistributes to mitotic structures like the spindle apparatus during M-phase. Functionally, MPHOSPH10 is implicated in ribosome biogenesis, cell proliferation, and proper chromosomal segregation. Structural studies suggest it contains multiple phosphorylation sites, enabling dynamic interactions with cell cycle regulators like CDK1/cyclin B complexes. Dysregulation of MPHOSPH10 expression is linked to genomic instability and tumorigenesis, with overexpression observed in several cancers, including hepatocellular carcinoma and glioblastoma.
Recombinant human MPHOSPH10 protein, typically produced in *E. coli* or mammalian expression systems, retains post-translational modifications when expressed in eukaryotic hosts. It serves as a tool to study mitotic mechanisms, screen anticancer drugs targeting cell cycle checkpoints, or investigate ribosome assembly pathways. Purification often involves affinity tags (e.g., His-tag) for efficient isolation. Recent studies also explore its role in viral infections, as some pathogens hijack MPHOSPH10 to manipulate host translation machinery. Further research aims to elucidate its dual roles in ribosome biogenesis and mitosis, potentially unveiling novel therapeutic targets.
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