| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOS |
| Uniprot No | P00540 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346 aa |
| 活性数据 | MPSPLALRPY LRSEFSPSVD ARPCSSPSEL PAKLLLGATL PRAPRLPRRL AWCSIDWEQV CLLQRLGAGG FGSVYKATYR GVPVAIKQVN KCTKNRLASR RSFWAELNVA RLRHDNIVRV VAASTRTPAG SNSLGTIIME FGGNVTLHQV IYGAAGHPEG DAGEPHCRTG GQLSLGKCLK YSLDVVNGLL FLHSQSIVHL DLKPANILIS EQDVCKISDF GCSEKLEDLL CFQTPSYPLG GTYTHRAPEL LKGEGVTPKA DIYSFAITLW QMTTKQAPYS GERQHILYAV VAYDLRPSLS AAVFEDSLPG QRLGDVIQRC WRPSAAQRPS ARLLLVDLTS LKAELG |
| 分子量 | 37.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MOS蛋白的3篇参考文献摘要概览：
1. **"Functional analysis of recombinant human MOS protein in meiotic resumption"**
*作者：Yamashita M 等*
摘要：研究团队成功在大肠杆菌中表达并纯化重组人MOS蛋白，证实其在卵母细胞中通过激活MAPK通路诱导减数分裂恢复，揭示了MOS在生殖细胞成熟中的关键调控作用。
2. **"Recombinant MOS kinase regulates cell cycle progression in somatic cells"**
*作者：Freeman RS 等*
摘要：通过体外实验证明重组人MOS蛋白具有丝氨酸/苏氨酸激酶活性，能够通过磷酸化下游靶点(如Cyclin B1)干扰有丝分裂进程，提示MOS异常表达可能导致基因组不稳定性。
3. **"Structural characterization of human MOS proto-oncogene product"**
*作者：Chen J 等*
摘要：利用昆虫细胞表达系统获得高纯度重组MOS蛋白，通过X射线晶体学解析其三维结构，发现N端激酶结构域存在独特的自抑制构象，为开发靶向MOS的肿瘤治疗策略提供结构基础。
注：MOS蛋白作为保守的丝氨酸/苏氨酸激酶，相关研究多聚焦于其在减数分裂启动、细胞周期调控和肿瘤发生中的双重作用。近年研究逐渐关注其重组表达系统的优化(如原核/真核表达体系比较)及病理机制探索。
Recombinant human MOS (Moloney murine sarcoma viral oncogene homolog) protein is a key player in cellular signaling and cell cycle regulation. MOS, a serine/threonine kinase encoded by the *MOS* proto-oncogene, belongs to the MAP kinase kinase kinase (MAP3K) family. It is primarily known for its role in meiosis and mitosis, particularly in oocyte maturation, where it activates the MAPK/ERK signaling cascade to trigger metaphase II arrest. Its expression is tightly regulated, with low levels in somatic cells but elevated activity in germ cells and certain cancers, linking it to oncogenic potential.
The recombinant form of MOS is produced via genetic engineering, often using bacterial (e.g., *E. coli*) or eukaryotic expression systems. This allows large-scale production of purified, bioactive protein for research. Recombinant MOS retains kinase activity, enabling studies on its interaction with downstream targets like mitogen-activated protein kinase kinase (MEK) and its role in cell cycle checkpoints. Researchers use it to dissect pathways involved in gametogenesis, embryogenesis, and cancer progression. Additionally, it serves as a tool for drug discovery, particularly in targeting hyperactive MOS signaling in malignancies. The recombinant protein’s reproducibility and high purity address challenges posed by low endogenous MOS levels in native tissues, facilitating mechanistic and structural studies.
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