| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MON1B |
| Uniprot No | Q7L1V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-547 aa |
| 活性数据 | MEVGGDTAAP APGGAEDLED TQFPSEEARE GGGVHAVPPD PEDEGLEETG SKDKDQPPSP SPPPQSEALS STSRLWSPAA PENSPTCSPE SSSGGQGGDP SDEEWRSQRK HVFVLSEAGK PIYSRYGSVE ALSATMGVMT ALVSFVQSAG DAIRAIYAED HKLVFLQQGP LLLVAMSRTS QSAAQLRGEL LAVHAQIVST LTRASVARIF AHKQNYDLRR LLAGSERTLD RLLDSMEQDP GALLLGAVRC VPLARPLRDA LGALLRRCTA PGLALSVLAV GGRLITAAQE RNVLAECRLD PADLQLLLDW VGAPAFAAGE AWAPVCLPRF NPDGFFYAYV ARLDAMPVCL LLLGTQREAF HAMAACRRLV EDGMHALGAM RALGEAASFS NASSASAPAY SVQAVGAPGL RHFLYKPLDI PDHHRQLPQF TSPELEAPYS REEERQRLSD LYHRLHARLH STSRPLRLIY HVAEKETLLA WVTSKFELYT CLSPLVTKAG AILVVTKLLR WVKKEEDRLF IRYPPKYSTP PATSTDQAAH NGLFTGL |
| 分子量 | 59.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MON1B蛋白的3篇假设性参考文献示例(请注意，这些文献为虚构示例，实际研究中请核实真实来源)：
1. **文献名称**：*"The MON1B-CCZ1 Complex Mediates Rab7 Activation in Endosomal Trafficking"*
**作者**：Zhang, Y. et al.
**摘要**：该研究通过在大肠杆菌中重组表达人MON1B与CCZ1蛋白，解析了二者形成的复合体在Rab7 GTP酶激活过程中的作用，揭示了其在晚期内体成熟及溶酶体功能调控中的机制。
2. **文献名称**：*"Recombinant Human MON1B Expression and Its Interaction with Membrane Lipids"*
**作者**：Kuroki, R. & Watanabe, S.
**摘要**：作者利用昆虫杆状病毒系统表达重组人MON1B蛋白，并通过脂质结合实验证明MON1B特异性地与磷脂酰肌醇-3-磷酸(PI3P)结合，为理解其在囊泡运输中的定位机制提供了依据。
3. **文献名称**：*"Structural Basis of MON1B-CCZ1 Heterodimer Assembly in Autophagosome-Lysosome Fusion"*
**作者**：Chen, L. et al.
**摘要**：通过冷冻电镜解析重组人MON1B-CCZ1复合体的三维结构，结合功能缺失实验，阐明该复合体在自噬体-溶酶体膜融合中的关键作用，为相关疾病治疗提供潜在靶点。
**提示**：若需真实文献，建议在PubMed或Google Scholar中以关键词“MON1B protein recombinant”、“human MON1B-Ccz1”检索，并筛选近年的功能或结构研究论文。
MON1B (Monensin sensitivity 1 homolog B), a member of the MON1 protein family, is a conserved eukaryotic protein involved in regulating intracellular membrane trafficking. It functions primarily in the endosomal-lysosomal system, partnering with CCZ1 to form the MON1-CCZ1 complex. This complex acts as a guanine nucleotide exchange factor (GEF) for Rab7. a small GTPase critical for late endosome maturation, autophagosome-lysosome fusion, and lysosomal biogenesis.
Human MON1B shares structural similarity with its paralog MON1A, featuring a LONGIN domain for membrane interaction and a conserved CCRP domain for Rab7 activation. Dysregulation of MON1B has been linked to lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s, where defective endolysosomal trafficking contributes to pathology. In cancer, MON1B overexpression may influence tumor progression through altered vesicle transport and growth signaling.
Recombinant human MON1B protein is typically produced via bacterial or mammalian expression systems, enabling biochemical studies of its Rab7 activation mechanism and screening for modulators. Its applications extend to deciphering membrane trafficking pathways, modeling lysosomal disorders, and developing therapeutics targeting autophagy-lysosomal dysfunction. Recent studies also suggest roles in immune cell functions and viral infection pathways involving endosomal escape. As membrane trafficking research expands, MON1B remains a key molecular player bridging vesicle dynamics to cellular homeostasis.
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