| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLXIPL |
| Uniprot No | Q9NP71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-217 aa |
| 活性数据 | MDEQGCEHTSRTEDPFIQPTDFGPSEPPLSVPPPISPVLPLVPPPATALNPPAPPTFHQPQKFAGVNKAPSVITHTASATLTHDAPATTFSQSQGLVITTHHPAPSAAPCGLALSPVTRPPQPRLTFVHPKPVSLTGGRPKQPHKIVPAPKPEPVSLVLKNARIAPGEPGGETQCGAPPDPEGCFPIPKAFKLVTTTTTLVCTCMRTHIHLNETKVS |
| 分子量 | 38.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人MLXIPL(ChREBP)蛋白相关的参考文献摘要信息：
1. **文献名称**: "Carbohydrate response element binding protein (ChREBP) regulates ribosomal protein biosynthesis in hepatocytes"
**作者**: Tong X, Zhao F, Mancuso A, et al.
**摘要**: 该研究利用重组ChREBP蛋白(MLXIPL)在肝癌细胞中的过表达，揭示了其通过调控核糖体蛋白合成影响肝细胞能量代谢的机制，强调了MLXIPL在糖代谢与细胞生长协同中的作用。
2. **文献名称**: "Structural basis for DNA recognition by the carbohydrate-responsive transcription factor ChREBP"
**作者**: Kay KL, Yamashita T, Krzysiak TC, et al.
**摘要**: 研究通过大肠杆菌系统重组表达并纯化人源ChREBP(MLXIPL)的DNA结合域，结合X射线晶体学解析了其与靶DNA结合的分子结构，阐明了MLXIPL在葡萄糖刺激下的DNA识别机制。
3. **文献名称**: "Recombinant ChREBP overexpression in adipocytes promotes lipid storage via interaction with PPARγ"
**作者**: Li Y, Kim JW, Park S, et al.
**摘要**: 团队在脂肪细胞中构建重组人ChREBP蛋白稳定表达模型，证明其通过激活PPARγ通路促进脂质储存，为MLXIPL在肥胖相关代谢疾病中的调控提供了实验依据。
*注：以上文献为示例性内容，实际引用需核实真实出版物数据。若需近期文献，建议通过PubMed或Web of Science以“recombinant MLXIPL/ChREBP”为关键词检索。*
**Background of Recombinant Human MLXIPL Protein**
MLXIPL (MLX interacting protein-like), also known as carbohydrate response element-binding protein (ChREBP), is a key transcriptional regulator involved in glucose and lipid metabolism. It belongs to the basic helix-loop-helix/leucine zipper (bHLH/Zip) family and forms heterodimers with MLX to activate target genes by binding to carbohydrate response elements (ChoREs) in their promoters. MLXIPL is primarily expressed in metabolic tissues, such as the liver, adipose tissue, and pancreas, where it mediates cellular responses to glucose availability by regulating genes like *PKLR* (pyruvate kinase) and *FASN* (fatty acid synthase).
Recombinant human MLXIPL protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional properties. This protein retains critical domains, including the glucose-sensing module and transcriptional activation domain, enabling research on its role in metabolic pathways. Dysregulation of MLXIPL is linked to metabolic disorders such as obesity, type 2 diabetes, and non-alcoholic fatty liver disease (NAFLD), making it a potential therapeutic target. Studies using recombinant MLXIPL have elucidated mechanisms of glucose sensing, lipogenesis, and crosstalk with insulin signaling pathways. Its application spans drug discovery, molecular interaction assays, and exploration of epigenetic regulation in metabolic homeostasis.
×
