| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLST8 |
| Uniprot No | Q9BVC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326 aa |
| 活性数据 | MNTSPGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNALEV TPDRSMIAAA GYQHIRMYDL NSNNPNPIIS YDGVNKNIAS VGFHEDGRWM YTGGEDCTAR IWDLRSRNLQ CQRIFQVNAP INCVCLHPNQ AELIVGDQSG AIHIWDLKTD HNEQLIPEPE VSITSAHIDP DASYMAAVNS TGNCYVWNLT GGIGDEVTQL IPKTKIPAHT RYALQCRFSP DSTLLATCSA DQTCKIWRTS NFSLMTELSI KSGNPGESSR GWMWGCAFSG DSQYIVTASS DNLARLWCVE TGEIKREYGG HQKAVVCLAF NDSVLG |
| 分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"mTOR Interacts with Raptor to Form a Nutrient-Sensitive Complex that Signals to the Cell Growth Machinery"**
- *Kim et al. (2002), Cell*
- 发现MLST8(GβL)是mTOR复合体1(mTORC1)的核心组分，与Raptor结合，共同调控细胞生长响应营养信号。
2. **"Mammalian target of rapamycin complex 2 regulates Akt kinase activity by phosphorylating the hydrophobic motif site"**
- *Sarbassov et al. (2005), Molecular and Cellular Biology*
- 揭示MLST8是mTORC2的关键结构蛋白，通过招募mTOR底物(如Akt)调控细胞存活和代谢。
3. **"Cryo-EM structure of the human mTOR complex 2"**
- *Yang et al. (2015), Science Advances*
- 通过冷冻电镜解析mTORC2的分子结构，显示MLST8的构象稳定作用及其与其他亚基的互作机制。
4. **"mTORC2 Activity Is Required for Adipose Tissue Lipid Homeostasis"**
- *Tang et al. (2019), Diabetes*
- 证明MLST8-mTORC2信号在脂肪细胞分化及脂代谢中至关重要，敲除MLST8导致胰岛素敏感性异常。
每篇均聚焦MLST8在mTOR复合体中的功能及其对下游通路的调控，涉及结构、代谢和疾病机制。
MLST8 (mammalian lethal with SEC13 protein 8), also known as GβL, is a critical regulatory component of the mammalian target of rapamycin (mTOR) signaling pathway. It serves as an essential scaffolding protein in three distinct complexes: mTOR complex 1 (mTORC1), mTORC2. and the GATOR2 complex (GSE). MLST8 stabilizes these complexes by mediating protein-protein interactions, particularly through its WD40 repeat domains. In mTORC1 and mTORC2. MLST8 directly binds to mTOR kinase, facilitating structural integrity and substrate recognition. While mTORC1 regulates cell growth in response to nutrients and energy status, mTORC2 controls cell survival and cytoskeletal organization. MLST8’s interaction with the GATOR2 complex further links it to amino acid sensing and mTORC1 regulation. Recombinant human MLST8 protein, produced via expression systems like E. coli or mammalian cells, retains these functional properties and is widely used in biochemical studies to dissect mTOR signaling mechanisms. Its role in nutrient-sensitive pathways and association with diseases such as cancer, diabetes, and neurological disorders make MLST8 a focal point for therapeutic research. Structural analyses highlight its conserved β-propeller architecture, which enables dynamic binding to multiple partners, positioning MLST8 as a versatile hub in cellular metabolism and growth control networks.
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