| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLPH |
| Uniprot No | Q9BV36 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600 aa |
| 活性数据 | MGKKLDLSKL TDEEAQHVLE VVQRDFDLRR KEEERLEALK GKIKKESSKR ELLSDTAHLN ETHCARCLQP YQLLVNSKRQ CLECGLFTCK SCGRVHPEEQ GWICDPCHLA RVVKIGSLEW YYEHVKARFK RFGSAKVIRS LHGRLQGGAG PELISEERSG DSDQTDEDGE PGSEAQAQAQ PFGSKKKRLL SVHDFDFEGD SDDSTQPQGH SLHLSSVPEA RDSPQSLTDE SCSEKAAPHK AEGLEEADTG ASGCHSHPEE QPTSISPSRH GALAELCPPG GSHRMALGTA AALGSNVIRN EQLPLQYLAD VDTSDEESIR AHVMASHHSK RRGRASSESQ IFELNKHISA VECLLTYLEN TVVPPLAKGL GAGVRTEADV EEEALRRKLE ELTSNVSDQE TSSEEEEAKD EKAEPNRDKS VGPLPQADPE VGTAAHQTNR QEKSPQDPGD PVQYNRTTDE ELSELEDRVA VTASEVQQAE SEVSDIESRI AALRAAGLTV KPSGKPRRKS NLPIFLPRVA GKLGKRPEDP NADPSSEAKA MAVPYLLRRK FSNSLKSQGK DDDSFDRKSV YRGSLTQRNP NARKGMASHT FAKPVVAHQS |
| 分子量 | 65.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人MLPH蛋白的参考文献及其摘要概述：
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1. **标题**: *"Melanophilin links Rab27a and myosin Va function in melanosome transport"*
**作者**: Hume et al. (2006)
**摘要**: 该研究揭示了MLPH在黑色素运输中的桥梁作用，证明其通过结合Rab27a和肌球蛋白Va协调黑素体在黑色素细胞内的运输。实验中利用重组人MLPH蛋白验证了其与两种蛋白的相互作用机制。
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2. **标题**: *"The Slac2-a/Melanophilin gene links to human pigmentary disorders"*
**作者**: Nagashima et al. (2002)
**摘要**: 作者通过表达重组人MLPH蛋白，分析了其与Rab27a的结构结合域，并发现某些MLPH突变与色素沉着疾病(如Griscelli综合征)相关，揭示其作为分子适配器的关键功能。
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3. **标题**: *"Role of MLPH in melanosome transport: Insights from in vitro reconstitution assays"*
**作者**: Van den Bossche et al. (2016)
**摘要**: 本研究利用重组人MLPH蛋白重建了黑素体运输的体外实验模型，证明MLPH的磷酸化修饰影响其与分子马达蛋白的亲和力，从而调控运输效率。
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4. **标题**: *"Structural and functional analysis of the ternary complex involving Rab27a, MLPH, and myosin Va"*
**作者**: Ivanova et al. (2019)
**摘要**: 通过X射线晶体学和重组人MLPH蛋白，解析了Rab27a-MLPH-肌球蛋白Va三元复合物的结构，阐明MLPH如何介导两者协同作用，推动黑素体沿微丝移动。
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以上文献均涉及重组人MLPH蛋白的功能与机制研究，涵盖结构分析、疾病关联及运输调控等领域。
Melanophilin (MLPH) is an adaptor protein critical for intracellular melanosome transport in melanocytes. It acts as a molecular bridge, linking Rab27a (a GTPase anchored on melanosomes) to myosin Va (a motor protein), facilitating melanosome movement along actin filaments to dendritic tips. This process enables melanin transfer to keratinocytes, influencing skin and hair color. MLPH mutations cause Griscelli syndrome type 3. characterized by silvery hair and mild pigmentation defects due to disrupted melanosome trafficking.
Structurally, MLPH contains an N-terminal Rab-binding domain and C-terminal regions mediating myosin interactions. Recombinant human MLPH, often produced in *E. coli* or mammalian cell cultures, retains these functional domains, allowing researchers to study its role in pigmentation pathways. It aids in elucidating pathological mechanisms of pigment disorders, testing therapeutic compounds, and analyzing mutation impacts via *in vitro* binding assays. Its applications extend to modeling melanocyte biology, exploring cosmetic interventions for pigmentation anomalies, and developing gene therapies for genetic syndromes. Recombinant MLPH thus serves as a key reagent in dermatological and cell biological research.
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