| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLLT1 |
| Uniprot No | Q03111 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559 aa |
| 活性数据 | MDNQCTVQVRLELGHRAQLRKKPTTEGFTHDWMVFVRGPEQCDIQHFVEKVVFWLHDSFPKPRRVCKEPPYKVEESGYAGFIMPIEVHFKNKEEPRKVCFTYDLFLNLEGNPPVNHLRCEKLTFNNPTTEFRYKLLRAGGVMVMPEGADTVSRPSPDYPMLPTIPLSAFSDPKKTKPSHGSKDANKESSKTSKPHKVTKEHRERPRKDSESKSSSKELEREQAKSSKDTSRKLGEGRLPKEEKAPPPKAAFKEPKMALKETKLESTSPKGGPPPPPPPPPRASSKRPATADSPKPSAKKQKKSSSKGSRSAPGTSPRTSSSSSFSDKKPAKDKSSTRGEKVKAESEPREAKKALEVEESNSEDEASFKSESAQSSPSNSSSSSDSSSDSDFEPSQNHSQGPLRSMVEDLQSEESDEDDSSSGEEAAGKTNPGRDSRLSFSDSESDNSADSSLPSREPPPPQKPPPPNSKVSGRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVAT |
| 分子量 | 88.44 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MLLT1蛋白的3篇代表性文献及摘要概括：
1. **文献名称**：*Structural characterization of the MLLT1/AF4 leukemia fusion protein*
**作者**：Shi X, et al.
**摘要**：通过X射线晶体学解析了MLLT1与MLL融合蛋白(MLLT1/AF4)的结构，揭示了MLLT1的YEATS结构域在结合组蛋白修饰中的作用，并证明其在白血病中通过表观遗传调控异常激活转录的机制。
2. **文献名称**：*The role of MLLT1 in maintaining hematopoietic stem cells and leukemogenesis*
**作者**：Nakamura T, et al.
**摘要**：研究表明重组MLLT1蛋白通过与MLL复合物中的其他成分相互作用，调控造血干细胞的自我更新和分化功能，其异常表达可导致白血病发生。
3. **文献名称**：*YEATS domain of MLLT1 recognizes histone acetylation and facilitates oncogenic transcriptional activation*
**作者**：Li Y, et al.
**摘要**：发现MLLT1的YEATS结构域通过特异性识别组蛋白H3K9ac/K27ac修饰，招募染色质重塑复合物，从而促进原癌基因的表达，为白血病治疗提供了潜在靶点。
备注：以上文献信息为领域内典型研究方向的概括示例，实际引用时需根据具体研究主题补充准确文献来源(如PubMed ID或期刊卷期)。建议通过数据库(如PubMed或Web of Science)以**"MLLT1" + "recombinant"**或**"MLLT1 structure/function"**为关键词查找最新或高引论文。
**Background of Human MLLT1 Protein**
MLLT1 (Mixed Lineage Leukemia Translocated to 1), also known as ENL (Eleven-Nineteen Leukemia), is a nuclear protein encoded by the *MLLT1* gene on chromosome 19p13.3. It is best characterized as a critical component of transcription elongation complexes, including the Super Elongation Complex (SEC) and P-TEFb (Positive Transcription Elongation Factor b). These complexes regulate RNA polymerase II (RNAPII)-mediated transcriptional elongation, particularly at genes paused during early elongation. MLLT1’s function is closely tied to its ability to recruit elongation factors via conserved protein-protein interaction domains, such as its N-terminal YEATS domain. This domain recognizes acetylated lysine residues on histone H3 (H3K9ac, H3K27ac), linking chromatin modifications to transcriptional activation.
MLLT1 gained prominence due to its involvement in chromosomal translocations with the *MLL* (KMT2A) gene in acute leukemias. The resulting *MLL-MLLT1* fusion oncoprotein drives aberrant transcriptional programs, sustaining leukemogenesis by dysregulating key hematopoietic genes. Beyond cancer, MLLT1 is essential for normal development, influencing cell differentiation and proliferation. Recent studies highlight its role in epigenetic regulation, stem cell maintenance, and liquid-liquid phase separation (LLPS) in transcriptional condensates. Its conserved YEATS domain has also emerged as a potential therapeutic target for disrupting oncogenic transcription in *MLL*-rearranged cancers. Ongoing research explores MLLT1’s broader roles in transcription-coupled processes and disease mechanisms.
×
