| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLF2 |
| Uniprot No | Q15773 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248 aa |
| 活性数据 | MFRFMRDVEP EDPMFLMDPF AIHRQHMSRM LSGGFGYSPF LSITDGNMPG TRPASRRMQQ AGAVSPFGML GMSGGFMDMF GMMNDMIGNM EHMTAGGNCQ TFSSSTVISY SNTGDGAPKV YQETSEMRSA PGGIRETRRT VRDSDSGLEQ MSIGHHIRDR AHILQRSRNH RTGDQEERQD YINLDESEAA AFDDEWRRET SRFRQQRPLE FRRLESSGAG GRRAEGPPRL AIQGPEDSPS RQSRRYDW |
| 分子量 | 28.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MLF2蛋白的3篇参考文献示例(注：以下内容为示例性虚构，实际文献需根据真实研究补充)：
---
1. **文献名称**：Structural and Functional Analysis of MLF2 in Leukemia Differentiation
**作者**：Chen L, et al.
**摘要**：该研究解析了MLF2蛋白的晶体结构，揭示了其通过调控核转运蛋白相互作用参与白血病细胞分化的分子机制，并利用重组MLF2验证了其在诱导细胞周期阻滞中的功能。
2. **文献名称**：Recombinant Human MLF2 Expression and Purification in E. coli
**作者**：Wang Y, et al.
**摘要**：本文开发了一种高效的大肠杆菌表达系统用于重组人MLF2蛋白生产，优化了可溶性和纯化流程，为后续功能研究提供高纯度蛋白来源。
3. **文献名称**：MLF2 Interaction with PML-NB Proteins in Tumor Suppression
**作者**：Saito M, et al.
**摘要**：研究发现MLF2与早幼粒白血病蛋白(PML)在核体内形成复合物，通过重组蛋白实验证明其能增强PML介导的肿瘤抑制通路，为癌症治疗提供新靶点。
4. **文献名称**：Role of MLF2 in DNA Damage Response
**作者**：Gupta R, et al.
**摘要**：通过重组MLF2蛋白及敲除模型，揭示了MLF2通过结合ATM激酶调控DNA损伤修复的分子机制，证明其缺失导致基因组不稳定性增加。
---
如需真实文献，建议通过PubMed、Web of Science等平台检索关键词“MLF2 protein”或“recombinant MLF2”。
**Background of Recombinant Human MLF2 Protein**
Myeloid leukemia factor 2 (MLF2) is a conserved eukaryotic protein implicated in transcriptional regulation, cellular differentiation, and apoptosis. Initially identified through its association with myeloid leukemia, MLF2 belongs to a protein family with homologs across species, suggesting essential roles in fundamental biological processes. Structurally, MLF2 contains conserved domains involved in protein-protein interactions, potentially linking it to chromatin remodeling or signal transduction pathways.
Recombinant human MLF2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified MLF2 for research. Its production enables the study of MLF2’s molecular mechanisms, including its role in hematopoiesis, cancer progression, and response to cellular stress. Studies suggest MLF2 interacts with nucleoporins and may influence nuclear transport or gene expression, though its precise functions remain under investigation.
In disease contexts, aberrant MLF2 expression is linked to leukemia, solid tumors, and developmental disorders, highlighting its therapeutic or diagnostic potential. Recombinant MLF2 serves as a critical tool for *in vitro* assays, structural studies, and drug screening, advancing understanding of its pathological and physiological roles. Ongoing research aims to clarify its regulatory networks and utility in biomedical applications.
×
