| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MKNK2 |
| Uniprot No | Q9HBH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465 aa |
| 活性数据 | MVQKKPAELQ GFHRSFKGQN PFELAFSLDQ PDHGDSDFGL QCSARPDMPA SQPIDIPDAK KRGKKKKRGR ATDSFSGRFE DVYQLQEDVL GEGAHARVQT CINLITSQEY AVKIIEKQPG HIRSRVFREV EMLYQCQGHR NVLELIEFFE EEDRFYLVFE KMRGGSILSH IHKRRHFNEL EASVVVQDVA SALDFLHNKG IAHRDLKPEN ILCEHPNQVS PVKICDFDLG SGIKLNGDCS PISTPELLTP CGSAEYMAPE VVEAFSEEAS IYDKRCDLWS LGVILYILLS GYPPFVGRCG SDCGWDRGEA CPACQNMLFE SIQEGKYEFP DKDWAHISCA AKDLISKLLV RDAKQRLSAA QVLQHPWVQG CAPENTLPTP MVLQRNSCAK DLTSFAAEAI AMNRQLAQHD EDLAEEEAAG QGQPVLVRAT SRCLQLSPPS QSKLAQRRQR ASLSSAPVVL VGDHA |
| 分子量 | 51.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MKNK2蛋白的3篇参考文献(基于虚拟生成示例，具体文献需根据实际检索补充)：
1. **文献名称**：*"Expression and functional characterization of recombinant human MKNK2 in cancer cell signaling"*
**作者**：Chen L, et al.
**摘要**：研究利用杆状病毒表达系统成功表达重组人MKNK2蛋白，并证实其通过磷酸化eIF4E调控mRNA翻译，促进肿瘤细胞增殖和耐药。
2. **文献名称**：*"Structural insights into the kinase activity of MKNK2 using recombinant protein crystallography"*
**作者**：Garcia-Ruiz E, et al.
**摘要**：通过大肠杆菌表达纯化重组MKNK2激酶结构域，解析其晶体结构，揭示ATP结合口袋特征，为靶向抑制剂设计提供依据。
3. **文献名称**：*"Role of recombinant MKNK2 in ERK/MAPK pathway modulation under oxidative stress"*
**作者**：Wang Y, et al.
**摘要**：在HEK293细胞中过表达重组MKNK2.证明其在氧化应激下通过ERK通路调控细胞凋亡，并验证特定磷酸化位点的功能。
(注：以上为示例性内容，实际文献需通过PubMed或学术数据库检索确认。)
Recombinant human MKNK2 (MAP kinase-interacting serine/threonine kinase 2) is a protein kinase involved in regulating mRNA translation and cellular stress responses. As a member of the Ser/Thr protein kinase family, MKNK2 interacts with the MAPK/ERK and p38 signaling pathways, acting downstream to phosphorylate substrates like eukaryotic translation initiation factor 4E (eIF4E). This phosphorylation influences cap-dependent translation, impacting processes such as cell proliferation, survival, and adaptation to environmental stressors. MKNK2 exists in two splice variants (MKNK2a and MKNK2b) with opposing roles in cancer: MKNK2a often acts as a tumor suppressor, while MKNK2b may promote oncogenesis, highlighting its context-dependent functionality.
Recombinant MKNK2 is produced using genetic engineering techniques, typically expressed in bacterial or mammalian systems to ensure proper post-translational modifications. Its recombinant form enables detailed biochemical studies, including kinase activity assays, structural analysis, and inhibitor screening. Researchers utilize recombinant MKNK2 to explore its dual roles in diseases, particularly cancer, and its potential as a therapeutic target. Dysregulation of MKNK2 has been linked to tumor progression, metastasis, and drug resistance, making it a focal point for developing kinase inhibitors or modulators of translation regulation. Ongoing studies aim to clarify its tissue-specific signaling mechanisms and therapeutic applications.
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