| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MKLN1 |
| Uniprot No | Q9UL63 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-735 aa |
| 活性数据 | AAGGAVAAA PECRLLPYAL HKWSSFSSTY LPENILVDKP NDQSSRWSSE SNYPPQYLIL KLERPAIVQN ITFGKYEKTH VCNLKKFKVF GGMNEENMTE LLSSGLKNDY NKETFTLKHK IDEQMFPCRF IKIVPLLSWG PSFNFSIWYV ELSGIDDPDI VQPCLNWYSK YREQEAIRLC LKHFRQHNYT EAFESLQKKT KIALEHPMLT DIHDKLVLKG DFDACEELIE KAVNDGLFNQ YISQQEYKPR WSQIIPKSTK GDGEDNRPGM RGGHQMVIDV QTETVYLFGG WDGTQDLADF WAYSVKENQW TCISRDTEKE NGPSARSCHK MCIDIQRRQI YTLGRYLDSS VRNSKSLKSD FYRYDIDTNT WMLLSEDTAA DGGPKLVFDH QMCMDSEKHM IYTFGGRILT CNGSVDDSRA SEPQFSGLFA FNCQCQTWKL LREDSCNAGP EDIQSRIGHC MLFHSKNRCL YVFGGQRSKT YLNDFFSYDV DSDHVDIISD GTKKDSGMVP MTGFTQRATI DPELNEIHVL SGLSKDKEKR EENVRNSFWI YDIVRNSWSC VYKNDQAAKD NPTKSLQEEE PCPRFAHQLV YDELHKVHYL FGGNPGKSCS PKMRLDDFWS LKLCRPSKDY LLRHCKYLIR KHRFEEKAQV DPLSALKYLQ NDLYITVDHS DPEETKEFQL LASALFKSGS DFTALGFSDV DHTYAQRTQL FDTLVNFFPD SMTPPKGNLV DLITL |
| 分子量 | 84.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MKLN1蛋白的模拟参考文献示例(文献信息为假设，供参考)：
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1. **文献名称**: *"Functional characterization of recombinant human MKLN1 in cell cycle regulation"*
**作者**: Zhang, Y. et al. (2018)
**摘要**: 本研究通过表达和纯化重组人MKLN1蛋白，揭示了其通过调控细胞周期蛋白(如Cyclin D1)参与细胞增殖的分子机制，提出MKLN1可能作为癌症治疗的潜在靶点。
2. **文献名称**: *"Structural analysis of MKLN1 reveals a ubiquitin-like domain critical for protein interaction"*
**作者**: Chen, L. et al. (2020)
**摘要**: 利用X射线晶体学解析了重组MKLN1蛋白的三维结构，发现其泛素样结构域与E3泛素连接酶的相互作用有关，为研究MKLN1介导的蛋白质降解通路提供了结构基础。
3. **文献名称**: *"MKLN1 modulates tau aggregation in neurodegenerative diseases"*
**作者**: Wang, H. et al. (2019)
**摘要**: 研究重组MKLN1蛋白在神经细胞中的作用，发现其通过与tau蛋白结合抑制异常聚集，提示MKLN1可能参与阿尔茨海默病的病理调控。
4. **文献名称**: *"Recombinant MKLN1 as a biomarker for breast cancer progression"*
**作者**: Li, X. et al. (2021)
**摘要**: 开发重组MKLN1蛋白作为检测工具，发现其在乳腺癌患者血清中显著高表达，并可能与肿瘤转移相关，提出其作为诊断标志物的潜力。
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**注**: 以上文献信息为模拟生成，实际研究中需通过PubMed、Web of Science等数据库检索真实文献。如需进一步协助定位特定研究，可提供更详细的关键词或研究方向。
The recombinant human MKLN1 (muskelin) protein is a engineered form of the naturally occurring MKLN1 protein, produced using recombinant DNA technology in laboratory systems such as bacterial, yeast, or mammalian cell cultures. MKLN1 is a cytosolic protein encoded by the *MKLN1* gene, characterized by multiple functional domains, including Kelch repeats and a BACK domain, which facilitate protein-protein interactions and intracellular scaffold functions. It plays a role in regulating endosomal trafficking, cytoskeletal organization, and cell signaling pathways, particularly those linked to neuronal development and cellular morphogenesis. Studies suggest its involvement in diseases like cancer and neurodegenerative disorders, though its precise mechanisms remain under investigation. Recombinant MKLN1 is purified for experimental use, enabling researchers to study its biochemical properties, interactions, and potential therapeutic applications. Its production allows controlled studies in vitro or in model organisms, aiding in the exploration of its role in vesicle transport, autophagy, and apoptosis. This tool is critical for advancing molecular and cellular research, drug discovery, and understanding disease pathogenesis linked to MKLN1 dysfunction.
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