| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIZF |
| Uniprot No | Q9BQA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517 aa |
| 活性数据 | MPPPGKVPRK ENLWLQCEWG SCSFVCSTME KFFEHVTQHL QQHLHGSGEE EEEEEEDDPL EEEFSCLWQE CGFCSLDSSA DLIRHVYFHC YHTKLKQWGL QALQSQADLG PCILDFQSRN VIPDIPDHFL CLWEHCENSF DNPEWFYRHV EAHSLCCEYE AVGKDNPVVL CGWKGCTCTF KDRSKLREHL RSHTQEKVVA CPTCGGMFAN NTKFLDHIRR QTSLDQQHFQ CSHCSKRFAT ERLLRDHMRN HVNHYKCPLC DMTCPLPSSL RNHMRFRHSE DRPFKCDCCD YSCKNLIDLQ KHLDTHSEEP AYRCDFENCT FSARSLCSIK SHYRKVHEGD SEPRYKCHVC DKCFTRGNNL TVHLRKKHQF KWPSGHPRFR YKEHEDGYMR LQLVRYESVE LTQQLLRQPQ EGSGLGTSLN ESSLQGIILE TVPGEPGRKE EEEEGKGSEG TALSASQDNP SSVIHVVNQT NAQGQQEIVY YVLSEAPGEP PPAPEPPSGG IMEKLQGIAE EPEIQMV |
| 分子量 | 59.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人MIZF蛋白的示例参考文献(注：由于MIZF研究较为小众，以下内容为学术假设性示例，建议通过学术数据库核实具体文献)：
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1. **标题**: *"Recombinant expression of human MIZF in mammalian cells and its role in chromatin remodeling"*
**作者**: Li X, et al.
**摘要**: 研究利用HEK293细胞表达重组人MIZF蛋白，并验证其通过锌指结构域与MBD2蛋白相互作用，调控组蛋白去乙酰化酶(HDAC)活性，影响染色质重塑过程。
2. **标题**: *"Purification and DNA-binding analysis of MIZF using a prokaryotic expression system"*
**作者**: Park S, Kim JH.
**摘要**: 描述在大肠杆菌中高效表达带His标签的重组MIZF蛋白，通过镍柱亲和层析纯化，并通过电泳迁移率变动实验(EMSA)证实其特异性结合甲基化CpG区域。
3. **标题**: *"MIZF knockdown inhibits tumor growth via p53 activation in glioblastoma models"*
**作者**: Gupta R, et al.
**摘要**: 通过重组MIZF蛋白功能缺失实验，发现MIZF通过抑制p53通路促进胶质母细胞瘤增殖，为靶向治疗提供潜在靶点。
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**建议**：若需真实文献，可访问PubMed或Web of Science，以“MIZF recombinant”、“MIZF protein interaction”为关键词检索，并筛选近年发表的研究。
**Background of Recombinant Human MIZF Protein**
Recombinant human MIZF (MYC-interacting zinc finger protein) is a protein engineered through genetic modification to mimic the native MIZF found in humans. MIZF, also known as ZNF802. belongs to the zinc finger protein family, characterized by conserved cysteine and histidine residues that form structural zinc-binding motifs. These motifs enable MIZF to interact with DNA, RNA, or other proteins, playing roles in transcriptional regulation and chromatin remodeling.
MIZF is particularly notable for its interaction with MYC, a proto-oncogene involved in cell proliferation, apoptosis, and metabolism. By binding to MYC, MIZF modulates its transcriptional activity, influencing pathways critical in cancer development and progression. Studies suggest MIZF acts as a tumor suppressor by inhibiting MYC-driven oncogenic processes, though its precise mechanisms remain under investigation.
Recombinant MIZF is typically produced in bacterial or mammalian expression systems, ensuring high purity and functionality for research applications. It serves as a vital tool for studying MYC regulatory networks, epigenetic modifications, and cancer biology. Additionally, its role in cellular stress responses and potential therapeutic targeting in malignancies highlights its biomedical relevance. Ongoing research aims to elucidate its broader functional landscape, including interactions with other signaling molecules and impact on disease pathologies.
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