| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIXL1 |
| Uniprot No | Q9H2W2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-232 aa |
| 活性数据 | MATAESRALQ FAEGAAFPAY RAPHAGGALL PPPSPAAALL PAPPAGPGPA TFAGFLGRDP GPAPPPPASL GSPAPPKGAA APSASQRRKR TSFSAEQLQL LELVFRRTRY PDIHLRERLA ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ PLARPEIILN HCAPGTETKC LKPQLPLEVD VNCLPEPNGV GGGISDSSSQ GQNFETCSPL SEDIGSKLDS WEEHIFSAFG NF |
| 分子量 | 24.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MIXL1蛋白的参考文献示例(注：文献为模拟生成，实际文献请通过学术数据库检索)：
1. **标题**：*MIXL1 regulates endoderm and mesoderm differentiation in human embryonic stem cells*
**作者**：Davis RP, et al.
**摘要**：研究通过重组MIXL1蛋白过表达，证明其在小鼠和人类胚胎干细胞向中胚层和内胚层分化中的关键作用，揭示了其与WNT/β-catenin通路的协同调控机制。
2. **标题**：*Recombinant MIXL1 protein enhances hematopoietic progenitor generation in vitro*
**作者**：Smith JR, et al.
**摘要**：团队利用重组MIXL1蛋白处理人诱导多能干细胞，显著提升了造血祖细胞的体外分化效率，为血液疾病模型构建提供新策略。
3. **标题**：*Purification and functional characterization of human MIXL1 protein for stem cell applications*
**作者**：Brown CM, et al.
**摘要**：首次报道重组MIXL1蛋白的高效纯化方法，验证其生物活性可通过调控靶基因如BRACHYURY的表达促进中胚层细胞分化。
**提示**：建议使用关键词“recombinant human MIXL1”或“MIXL1 protein stem cell differentiation”在PubMed或Google Scholar中检索最新文献，重点关注其实验应用(如细胞重编程或疾病建模)。
Mix1-like protein 1 (MIXL1) is a transcription factor belonging to the Mix family of homeobox genes, which play crucial roles in embryonic development. Initially identified through homology with the Xenopus Mix.1 gene, human MIXL1 is involved in early cell fate determination, particularly in the formation of mesodermal and endodermal lineages during gastrulation. It regulates the expression of downstream target genes by binding to specific DNA sequences, thereby influencing cellular differentiation and tissue patterning.
MIXL1 is essential for maintaining the pluripotency of embryonic stem cells (ESCs) and directing their differentiation into specialized cell types. Studies link its activity to key signaling pathways like Wnt, BMP, and Notch, which orchestrate developmental processes. Dysregulation of MIXL1 has been implicated in various pathologies, including hematopoietic disorders and cancers such as leukemia, where aberrant expression may disrupt normal cell maturation.
Recombinant human MIXL1 protein, produced via engineered expression systems (e.g., E. coli or mammalian cells), serves as a vital tool for functional studies. It enables researchers to explore its role in stem cell differentiation, model developmental pathways in vitro, and screen therapeutic agents targeting MIXL1-associated diseases. Purified recombinant MIXL1 retains DNA-binding activity, making it suitable for assays like electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP). Ongoing research focuses on harnessing its regulatory potential for regenerative medicine and precision oncology.
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