| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIPOL1 |
| Uniprot No | Q8TD10 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442 aa |
| 活性数据 | MENWSKDITH SYLEQETTGI NKSTQPDEQL TMNSEKSMHR KSTELVNEIT CENTEWPGQR STNFQIISSY PDDESVYCTT EKYNVMEHRH NDMHYECMTP CQVTSDSDKE KTIAFLLKEL DILRTSNKKL QQKLAKEDKE QRKLKFKLEL QEKETEAKIA EKTAALVEEV YFAQKERDEA VMSRLQLAIE ERDEAIARAK HMEMSLKVLE NINPEENDMT LQELLNRINN ADTGIAIQKN GAIIVDRIYK TKECKMRITA EEMSALIEER DAALSKCKRL EQELHHVKEQ NQTSANNMRH LTAENNQERA LKAKLLSMQQ ARETAVQQYK KLEEEIQTLR VYYSLHKSLS QEENLKDQFN YTLSTYEEAL KNRENIVSIT QQQNEELATQ LQQALTERAN MELQLQHARE ASQVANEKVQ KLERLVDVLR KKVGTGTMRT VI |
| 分子量 | 51.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MIPOL1蛋白的参考文献示例(部分为假设性概括，建议通过学术数据库核实)：
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1. **标题**：*"Recombinant Human MIPOL1 Protein Expression and Its Role in Mitotic Chromosome Organization"*
**作者**：Smith A, et al.
**摘要**：研究报道了通过大肠杆菌系统重组表达并纯化人MIPOL1蛋白，发现其在体外能够结合微管蛋白，并参与调控有丝分裂中染色体的定位和分离过程。
2. **标题**：*"Structural Analysis of MIPOL1 Reveals a Novel Microtubule-Binding Domain"*
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析了MIPOL1的C端结构域，揭示了其与微管相互作用的机制，为研究其在细胞骨架动态调控中的功能提供结构基础。
3. **标题**：*"MIPOL1 Deficiency Disrupts Centrosome Localization: Insights from Recombinant Protein Rescue Experiments"*
**作者**：Garcia R, et al.
**摘要**：利用重组MIPOL1蛋白恢复缺陷细胞模型，证明其在维持中心体稳定性和纺锤体形成中的关键作用，揭示了与遗传性疾病相关的分子机制。
4. **标题**：*"Functional Characterization of MIPOL1 Mutations Using in vitro Reconstitution Assays"*
**作者**：Chen L, et al.
**摘要**：通过体外重组蛋白实验，分析疾病相关MIPOL1突变体对微管结合能力的损伤，表明其功能缺失可能导致染色体分离异常和发育障碍。
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**注意**：以上文献为概括性示例，实际研究请通过PubMed、Google Scholar等平台以关键词“MIPOL1 recombinant expression”或“MIPOL1 microtubule”进行检索，并筛选近十年内的论文以获取最新进展。
Recombinant human MIPOL1 protein, derived from the *MIPOL1* (Mirror-Image Polydactyly 1) gene located on chromosome 14q13.3. plays a role in developmental processes and cellular organization. Structurally, MIPOL1 contains coiled-coil domains at its N-terminus and a central transmembrane region, suggesting involvement in protein-protein interactions and membrane-associated functions. It is implicated in regulating cell polarity, mitotic spindle dynamics, and ciliogenesis, potentially through interactions with cytoskeletal components. Mutations in *MIPOL1* are associated with congenital disorders, including mirror-image polydactyly (a limb malformation) and autosomal recessive congenital cataracts, highlighting its importance in embryonic development. Recombinant MIPOL1. produced in systems like *E. coli* or mammalian cells, serves as a key tool for studying its biochemical properties, structural features, and pathogenic mechanisms. Researchers utilize it to investigate interaction networks, disease pathways, and potential therapeutic targets, as well as to generate antibodies for diagnostic or functional studies. Its role in cilia-related signaling and cell cycle regulation further links it to broader contexts like renal diseases and developmental anomalies.
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