| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIER1 |
| Uniprot No | Q8N108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512 aa |
| 活性数据 | MAEPSVESSS PGGSATSDDH EFDPSADMLV HDFDDERTLE EEEMMEGETN FSSEIEDLAR EGDMPIHELL SLYGYGSTVR LPEEDEEEEE EEEEGEDDED ADNDDNSGCS GENKEENIKD SSGQEDETQS SNDDPSQSVA SQDAQEIIRP RRCKYFDTNS EVEEESEEDE DYIPSEDWKK EIMVGSMFQA EIPVGICRYK ENEKVYENDD QLLWDPEYLP EDKVIIFLKD ASRRTGDEKG VEAIPEGSHI KDNEQALYEL VKCNFDTEEA LRRLRFNVKA AREELSVWTE EECRNFEQGL KAYGKDFHLI QANKVRTRSV GECVAFYYMW KKSERYDFFA QQTRFGKKKY NLHPGVTDYM DRLLDESESA ASSRAPSPPP TASNSSNSQS EKEDGTVSTA NQNGVSSNGP GEILNKEEVK VEGLHINGPT GGNKKPLHAD MDTNGYETDN LTTDPKLAHM TARNENDFDE KSERPAKRRR VNSNGKESPG SSEFFQEAVS HGKFEELENT DD |
| 分子量 | 57.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MIER1蛋白的3篇文献示例，基于现有研究方向的合理推测整理：
1. **文献名称**: "Cloning, Expression, and Functional Analysis of the Recombinant Human MIER1 Protein"
**作者**: Smith J, et al.
**摘要**: 该研究描述了人MIER1基因的克隆，通过大肠杆菌系统成功表达并纯化了重组MIER1蛋白。功能实验表明，该蛋白可通过结合特定DNA序列抑制靶基因转录，提示其在表观遗传调控中的作用。
2. **文献名称**: "MIER1 Interaction with Histone Deacetylase 1 (HDAC1) Modulates Cell Cycle Progression"
**作者**: Lee S, et al.
**摘要**: 利用重组MIER1蛋白进行体外结合实验，发现其与HDAC1直接相互作用。此复合物通过去乙酰化作用调控组蛋白修饰，影响细胞周期相关基因表达，进而抑制乳腺癌细胞的增殖。
3. **文献名称**: "Structural Insights into MIER1’s Transcriptional Repression Domain"
**作者**: Zhang R, et al.
**摘要**: 通过X射线晶体学解析重组MIER1蛋白C端结构，鉴定出关键的α-螺旋结构域，该区域对其招募染色质修饰酶(如HDACs)至关重要，为MIER1在基因沉默中的作用提供结构基础。
**注意事项**：
以上文献为假设性示例，实际研究中MIER1相关重组蛋白的专门报道较少。建议通过PubMed或Google Scholar以“recombinant MIER1 protein”“MIER1 structure/function”等关键词检索最新文献。MIER1的研究多聚焦于其转录抑制功能及在癌症(如乳腺癌、结直肠癌)中的抑癌机制。
MIER1 (Mesoderm Induction Early Response 1) is a protein encoded by the *MIER1* gene, belonging to the MIER family of transcriptional regulators. Initially identified for its role in mesoderm induction during early embryonic development, MIER1 has since been linked to diverse cellular processes, including chromatin remodeling, transcriptional regulation, and signal transduction. Structurally, it contains several conserved domains, such as an acidic transcriptional activation domain, a nuclear localization signal, and an LXXLL motif that mediates interactions with nuclear receptors. Additionally, certain isoforms may possess a chromodomain or PHD finger, suggesting involvement in epigenetic modifications by recruiting histone deacetylases (HDACs) or other chromatin-modifying complexes.
Functionally, MIER1 acts as a transcriptional co-regulator, capable of both activating and repressing target genes depending on cellular context. It interacts with HDAC1/2-containing complexes to modulate chromatin structure and gene expression, influencing processes like cell differentiation, proliferation, and apoptosis. Studies implicate MIER1 in cancer biology, where it may serve dual roles as a tumor suppressor or oncogene. For example, reduced MIER1 expression correlates with poor prognosis in certain cancers, while overexpression has been observed in others. Its activity is regulated by post-translational modifications (e.g., phosphorylation) and alternative splicing, generating isoforms (MIER1α and MIER1β) with opposing effects on transcription. Recombinant MIER1 proteins are widely used to dissect its molecular mechanisms, including DNA-binding specificity, protein interactions, and regulatory pathways like Wnt/β-catenin or TGF-β signaling. Ongoing research seeks to clarify its tissue-specific roles and therapeutic potential.
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