| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MICAL3 |
| Uniprot No | Q7RTP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-948 aa |
| 活性数据 | MEERKHETMNPAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLIQPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNINVNFLRPSQVRHLYDTGETKDIHLEMESLVNSRTTPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLPSSDTLDLNAEEKAVLIASTRSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEEAPRGHRGERPTLVSTLTDRRMDVAVGNQNKVKYMATQLLAKFEENAPAQSIGIRRQGSMKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDGKFYCKPHYCYRLSGYAQRKRPAVAPLSGKEAKGPLQDGATTDANGRANAVASSTERTPGSGVNGLEEPSIAKRLRGTPERIELENYRLSLRQAEALQEVPEETQAEHNLSSVLDTGAEEDVASRSARRAAGRPPATRP |
| 分子量 | 130.68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MICAL3蛋白相关的模拟参考文献(示例)，实际文献需通过学术数据库检索：
1. **文献名称**: "Structural insights into MICAL3-mediated actin filament disassembly through redox modification"
**作者**: Smith A, et al.
**摘要**: 解析了重组人MICAL3蛋白的氧化酶结构域晶体结构，揭示了其通过产生活性氧(ROS)氧化修饰肌动蛋白微丝，调控细胞骨架动态的分子机制。
2. **文献名称**: "MICAL3 interacts with Rab GTPases to modulate vesicular trafficking in cancer cells"
**作者**: Li J, et al.
**摘要**: 发现重组MICAL3蛋白与Rab家族小GTP酶结合，通过调控内体运输促进肿瘤细胞迁移，提示其在癌症转移中的潜在作用。
3. **文献名称**: "MICAL3 regulates autophagosome-lysosome fusion via oxidation of microtubule-associated protein EB1"
**作者**: Wang Y, et al.
**摘要**: 证明重组MICAL3通过氧化EB1蛋白破坏微管稳定性，从而抑制自噬体-溶酶体融合，影响细胞自噬过程。
4. **文献名称**: "Functional characterization of MICAL3 in neuronal development using CRISPR/Cas9 and recombinant protein assays"
**作者**: Chen L, et al.
**摘要**: 利用重组MICAL3蛋白及基因编辑技术，发现其通过Semaphorin信号通路调控轴突导向和神经元极化。
**注意**：以上内容为模拟生成，具体文献需参考PubMed、Google Scholar等数据库，并核实作者及摘要准确性。
MICAL3 (Molecular Interacting with CasL 3) is a member of the MICAL family of cytoskeleton-regulatory proteins, known for their roles in redox enzymatic activity and cytoskeletal dynamics. As a flavoprotein monooxygenase, MICAL3 contains a conserved N-terminal flavin-binding domain that facilitates redox-dependent modifications of cytoskeletal components, particularly actin filaments. It interacts with Rab GTPases and other signaling molecules to regulate membrane trafficking, organelle positioning, and cell polarization.
Expressed predominantly in vertebrates, MICAL3 localizes to intracellular vesicles and the Golgi apparatus, where it influences microtubule stability and actin network remodeling. Its redox activity promotes actin depolymerization through methionine oxidation, impacting cell motility and morphogenesis. Recombinant human MICAL3 protein is typically expressed in eukaryotic systems (e.g., HEK293 or insect cells) to preserve post-translational modifications and enzymatic activity.
Studies implicate MICAL3 in cancer progression, neuronal development, and cardiovascular diseases, with dysregulation linked to metastatic invasion and axonal guidance defects. Structural and functional analyses of recombinant MICAL3 aim to elucidate its role in cellular signaling and explore therapeutic targeting of its redox-active domain. Its interplay with cytoskeletal and trafficking pathways highlights its potential as a multifaceted regulator of cell behavior.
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