| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIA2 |
| Uniprot No | Q96PC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-542 aa |
| 活性数据 | TKLLADLK KCGDLECEAL INRVSAMRDY RGPDCRYLNF TKGEEISVYV KLAGEREDLW AGSKGKEFGY FPRDAVQIEE VFISEEIQMS TKESDFLCLL GVSYTFDNED SELNGDYGEN IYPYEEDKDE KSSIYESDFQ IEPGFYATYE STLFEDQVPA LEAPEDIGST SESKDWEEVV VESMEQDRIP EVHVPPSSAV SGVKEWFGLG GEQAEEKAFE SVIEPVQESS FRSRKIAVED ENDLEELNNG EPQTEHQQES ESEIDSVPKT QSELASESEH IPKPQSTGWF GGGFTSYLGF GDEDTGLELI AEESNPPLQD FPNSISSDKE ATVPCTEILT EKKDTITNDS LSLKPSWFDF GFAILGFAYA KEDKIMLDDR KNEEDGGADE HEHPLTSELD PEKEQEIETI KIIETEDQID KKPVSEKTDE SDTIPYLKKF LYNFDNPWNF QNIPKETELP FPKQILDQNN VIENEETGEF SIDNYPTDNT KVMIFKSSYS LSGWYENIYI RIYFIFKHNL FL |
| 分子量 | 159.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MIA2蛋白的3条参考文献示例(注：内容为虚构，仅供格式参考)：
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1. **文献名称**：*Recombinant Human MIA2 Protein: Expression in E. coli and Functional Characterization*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统高效表达了可溶性重组人MIA2蛋白，利用亲和层析纯化获得高纯度蛋白。实验证实MIA2能抑制黑色素瘤细胞的迁移，并通过整合素通路调控细胞黏附。
2. **文献名称**：*Structural Insights into MIA2 Interaction with Extracellular Matrix Components*
**作者**：Li X, Wang Y.
**摘要**：采用X射线晶体学解析了MIA2的蛋白结构，揭示其SH3结构域与胶原蛋白IV的结合机制，为MIA2在组织重塑中的功能提供了分子基础。
3. **文献名称**：*MIA2 as a Serum Biomarker in Hepatocellular Carcinoma*
**作者**：Garcia R, et al.
**摘要**：通过ELISA检测发现，肝癌患者血清中MIA2水平显著升高，与肿瘤侵袭性正相关，提示MIA2或可作为预后标志物及治疗靶点。
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如需真实文献，请通过PubMed或Google Scholar检索关键词“recombinant MIA2 protein”、“MIA2 function”等。
Recombinant human Melanoma Inhibitory Activity-2 (MIA2) protein, also known as transport and Golgi organization protein 1 (TANGO1c), is a secreted member of the MIA/OTOR family. It shares structural homology with MIA1. including a conserved SH3-like domain and a unique C-terminal region. MIA2 is primarily expressed in the liver and adipose tissue, distinguishing it from MIA1's predominant expression in cartilage and melanoma. Its biological functions involve interactions with extracellular matrix components and modulation of cellular processes like adhesion, migration, and signaling pathways.
Produced through recombinant DNA technology in systems like E. coli or mammalian cells, recombinant MIA2 maintains its native conformation and functionality while allowing scalable production. The protein's SRCR (scavenger receptor cysteine-rich) domain facilitates molecular interactions, particularly with collagen types, influencing tissue remodeling and cellular communication. Clinically, MIA2 has emerged as a potential biomarker in hepatocellular carcinoma and colorectal cancer, with studies suggesting its role in tumor progression through regulation of cell invasion and metastasis. Recent research also implicates MIA2 in metabolic regulation, including lipid metabolism modulation and hepatic stress response, highlighting its therapeutic potential for metabolic disorders. Current investigations focus on its precise molecular mechanisms and diagnostic applications in chronic liver diseases.
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