| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGRN1 |
| Uniprot No | O60291 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-552 aa |
| 活性数据 | GSILSRRIA GVEDIDIQAN SAYRYPPKSG NYFASHFFMG GEKFDTPHPE GYLFGENMDL NFLGSRPVQF PYVTPAPHEP VKTLRSLVNI RKDSLRLVRY KDDADSPTED GDKPRVLYSL EFTFDADARV AITIYCQASE EFLNGRAVYS PKSPSLQSET VHYKRGVSQQ FSLPSFKIDF SEWKDDELNF DLDRGVFPVV IQAVVDEGDV VEVTGHAHVL LAAFEKHMDG SFSVKPLKQK QIVDRVSYLL QEIYGIENKN NQETKPSDDE NSDNSNECVV CLSDLRDTLI LPCRHLCLCT SCADTLRYQA NNCPICRLPF RALLQIRAVR KKPGALSPVS FSPVLAQSLE HDEHSCPFKK SKPHPASLAS KKPKRETNSD SVPPGYEPIS LLEALNGLRA VSPAIPSAPL YEEITYSGIS DGLSQASCPL AAIDHILDSS RQKGRPQSKA PDSTLRSPSS PIHEEDEEKL SEDVDAPPPL GGAELALRES SSPESFITEE VDESSSPQQG TRAASIENVL QDSSPEHCGR GPPADIYLPA LGPDSCSVGI DE |
| 分子量 | 60.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MGRN1蛋白的3篇文献示例：
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1. **文献名称**：*MGRN1 as a phenotypic modifier of sequestration in degenerative neuropathies: A study in Charcot-Marie-Tooth disease*
**作者**：A. H. Bhat et al.
**摘要**：探讨MGRN1通过调控泛素-蛋白酶体系统在神经退行性疾病中的作用，发现重组人MGRN1蛋白可减少突变蛋白聚集，缓解Charcot-Marie-Tooth病模型中的病理表型。
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2. **文献名称**：*Mahogunin ring finger 1 promotes transient receptor potential channel polyubiquitination and degradation*
**作者**：L. Chen et al.
**摘要**：研究重组人MGRN1对TRP通道的调控机制，证明其通过E3泛素连接酶活性介导TRPV4蛋白的泛素化及降解，影响细胞应激响应。
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3. **文献名称**：*Role of MGRN1 in prion protein trafficking: Insights from recombinant protein expression*
**作者**：S. K. Singh & T. Nakamura
**摘要**：利用重组MGRN1蛋白揭示其与朊蛋白(PrP)的相互作用机制，表明MGRN1通过调控内体-溶酶体通路影响PrP的胞内运输与错误折叠。
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注：以上文献信息为示例性内容，实际引用时请通过学术数据库(如PubMed、Web of Science)核实具体文献。
Mahogunin Ring Finger 1 (MGRN1) is a member of the RING finger family of E3 ubiquitin ligases, which plays a critical role in ubiquitin-mediated protein degradation and cellular homeostasis. First identified through its association with the mahoganoid coat color mutation in mice, MGRN1 is ubiquitously expressed in mammalian tissues, particularly enriched in the brain, testes, and melanocytes. Structurally, it contains a conserved C-terminal RING domain essential for its enzymatic activity and a unique N-terminal region implicated in substrate recognition and intracellular trafficking.
MGRN1 regulates diverse biological processes, including melanosome biogenesis, lysosomal function, and mitochondrial dynamics. It interacts with proteins like TSG101 and alpha-synuclein, influencing endosomal sorting and preventing protein aggregation. Notably, MGRN1 dysfunction is linked to neurodegenerative diseases such as Alzheimer's and Parkinson's, where impaired ubiquitination contributes to pathogenic protein accumulation. In melanocytes, MGRN1 deficiency disrupts pigmentation by altering tyrosinase trafficking, while its role in spermatogenesis highlights broader implications for fertility.
Recent studies also suggest its involvement in apoptosis and autophagy regulation. Despite progress, mechanistic details of substrate specificity and tissue-specific functions remain underexplored, making MGRN1 a focus of ongoing research in cell biology and disease pathogenesis.
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